| ²é¿´: 2192 | »Ø¸´: 10 | |||
| µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû | |||
[½»Á÷]
Î÷±±Å©ÁֿƼ¼´óѧ Nat Commun ·¢²¼Ð¿Ö¸´ò°ÐÉú²ú¿¹²¡×ª»ùÒòţвßÂÔ
|
|||
|
À´×ÔÎ÷±±Å©ÁֿƼ¼´óѧµÄÑо¿ÈËÔ±³É¹¦ÀûÓÃпָÇпÚø£¨zinc-finger nickases,ZFN£©½éµ¼½«ÈÜÆÏÌÑÇò¾úËØ£¨lysostaphin£©»ùÒò²åÈëµ½¦Â-ÀÒµ°°×£¨beta-casein£©»ùÒò×ù£¬Óɴ˹¹½¨³öÁË»î²ú»ùÒò°ÐÏòÐÔ¿Ë¡ţ¡£ÕâÒ»Í»ÆÆÐÔµÄÑо¿³É¹û·¢±íÔÚ10ÔÂ14Èյġ¶×ÔȻͨѶ¡·£¨Nature Communications£©ÔÓÖ¾ÉÏ¡£ Áìµ¼ÕâÒ»Ñо¿µÄÊÇÎ÷±±Å©ÁֿƼ¼´óѧµÄÕÅÓ¿£¨Yong Zhang£©½ÌÊÚ£¬Æä¶àÄê´Óʲ¸È鶯Îï·¢ÓýÉúÎ﹤³ÌÀíÂۺͼ¼ÊõÑо¿¡£Êǹú¼Ò¼¶¡°ÓÐÍ»³ö¹±Ï×ר¼Ò¡±¡£ ZFNÊÇÒ»ÖÖÊÊÓÃÓÚ»ùÒò×鹤³Ì²Ù×Ý¡¢ÊµÏÖ¶¨ÏòÍ»±äµÄÇ¿ÓÐÁ¦µÄ¹¤¾ß¡£ZFNsÇиîDNA£¬ÔÚ»ùÒò×éÖÐÉú³ÉλµãÌØÒìÐÔË«Á´¶ÏÁÑ(DSBs)¡£»òͨ¹ý·ÇͬԴĩ¶ËÁ¬½Ó(NHEJ)ÐÞ¸´Ë«Á´¶ÏÁÑ£¬ÍùÍù»áÔÚÁ¬½Ó¶Ïµãµ¼Èë¶ÌµÄ²åÈë»òȱʧ(indels)£¬»òÊÇͨ¹ýÍ¬Ô´ÖØ×éÐÞ¸´£¨HDR£©¿½±´½ãÃÃȾɫµ¥ÌåÖÒʵµØÐÞ¸´ÔʼÐòÁС£¾¡¹Ü·ÇͬԴĩ¶ËÁ¬½ÓÊǸߵÈÕæºËϸ°ûºÍÉúÎïÌåÖ÷ÒªµÄË«Á´¶ÏÁÑÐÞ¸´ÐźÅͨ·£¬Ä¿Ç°Ñо¿ÈËÔ±ÀûÓÃÁËÕâÁ½ÖÖÐźÅͨ·À´ÊµÏÖ¶¨µã»ùÒò×éÐÞÊΡ£ µ±Ç°£¬ZFNs±»¹ã·ºµØÓ¦ÓÃÓÚ»ù´¡Ñо¿¡¢ÉúÎï¼¼ÊõºÍҽѧÁìÓò£¬µ«ÓÉÓÚ²»¿É±ÜÃâµØÉú³ÉË«Á´¶ÏÁÑÒÔ¼°·ÇͬԴĩ¶ËÁ¬½ÓµÄÒ׳ö´íÐÔ£¬ÀûÓÿɱà³ÌºËËáø½øÐлùÒò×鹤³Ì²Ù×ÝÊܵ½ÏÞÖÆ¡£²¢ÇÒ£¬ÕâЩ¿É±à³ÌºËËáøÍùÍù»áÔڸ߶ÈͬԴµÄλµãÓÕµ¼³öËæ»úÉú³É¼°²»ÐèÒªµÄȱʧ¡£¶øÇÒºËËáøÓÕµ¼Ì«¶àÍѰÐË«Á´¶ÏÁѶÔϸ°ûÓк¦¡£´ËÍ⣬ҪÕýÈ·µØ·ÖÀë³ö»ùÒò°ÐÏòϸ°ûÒ²²»ÊǼþÈÝÒ×µÄÊÂÇé¡£ ¿Ë·þÕâЩ¾ÖÏÞµÄÒ»ÖÖDZÔÚ²ßÂÔ¾ÍÊÇ£¬°ÐÏòÐÔµ¼Èëµ½°üº¬Ò»¸öµ¥Á´¶ÏÁÑ£¨SSB£©»òÇпڵÄDNAÖС£ÏñË«Á´¶ÏÁÑÒ»Ñù£¬µ¥Á´¶ÏÁÑ/ÇпÚÀíÂÛÉÏ¿ÉÒԴ̼¤Í¬Ô´ÖØ×éÐÞ¸´ÐźÅͨ·¡£ÓëË«Á´¶ÏÁÑÏà±È£¬ÇпڻòSSB²»ÊÇ·ÇͬԴĩ¶ËÐÞ¸´µÄµ×Îï¡£Òò´Ë£¬°ÐÏòÐÔSSBÓÐDZÁ¦½«ÐÞ¸´ÏÞ¶¨ÖÁÍ¬Ô´ÖØ×éÐÞ¸´ÐźÅͨ·¡£ ÈÜÆÏÌÑÇò¾úËØ¿ÉÓÉÈÜѪÐÔÆÏÌÑÇò¾ú×ÔÈ»Éú³É£¬ÒÑÓÐÈËÌá³öÓÃËüÀ´½øÐÐÈ«ÉíÐÔÖÎÁÆÈÜѪÐÔÆÏÌÑÇò¾ú¸ÐȾ¡£ÔÚСÊóÄ£ÐÍÖУ¬ÈÜÆÏÌÑÇò¾úËØÒѱ»Ö¤ÊµÄܹ»¶Ô¿¹ÈÜѪÐÔÆÏÌÑÇò¾úÒýÆðµÄÈéÏÙÑס£ÓÐÑо¿±¨µÀ£¬ÈéÖÖбí´ïÈÜÆÏÌÑÇò¾úËØµÄת»ùÒòÅ£Äܹ»µÖ¿¹ÈÜѪÐÔÆÏÌÑÇò¾úÈé·¿¸ÐȾ¡£È»¶øÔÚÌåÍâ²ÉÓò¸È鶯Îïϸ°ûÉú³ÉµÄÈÜÆÏÌÑÇò¾úËØÓÉÓÚÌÇ»ù»¯ÓпÉÄÜ·¢Éúµ°°×ÖÊʧ»î¡£Òò´Ë£¬Ð޸ıàÂëÐòÁÐÉú³ÉGln125,232-ÈÜÆÏÌÑÇò¾úËØÈ·±£ÁËÁ½¸öDZÔÚÌÇ»ù»¯Î»µã±»ÒƳý¡£Õâ¿ÉÒÔ·ÀÖ¹µ°°×ÖÊÌÇ»ù»¯²¢»Ö¸´²¿·Öµ°°×ÖÊ»îÐÔ¡£ ÔÚÐÂÑо¿ÖУ¬Ñо¿ÈËÔ±µÄÄ¿µÄÖ¼ÔÚͨ¹ýÔÚÅ£ÅßÌ¥³ÉÏËάϸ°ûÄÚ½«ÍâÔ´ÐÔGln125,232ÈÜÆÏÌÑÇò¾úËØ»ùÒò²åÈëµ½¦Â-ÀÒµ°°×»ùÒò×ùÖУ¬½øÐÐÓÐЧ¡¢¿ÉÖØÏֵĻùÒò°ÐÏò£¬´Ó¶øÊ¹µÃ»ùÒò°ÐÏòÅ£µÄÈéÏÙÄܹ»Éú³ÉÈÜÆÏÌÑÇò¾úËØµ°°×¡£ Ñо¿ÈËÔ±Ëæºó֤ʵÁË£¬ÔÚÅ£ÅßÌ¥³ÉÏËάϸ°ûÖн«ZFNickases°ÐÏòÄÚÔ´¦Â-ÀÒµ°°×»ùÒò×ù£¬Í¨¹ýÍ¬Ô´ÖØ×éÐÞ¸´´Ì¼¤¿ÉÌí¼ÓÈÜÆÏÌÑÇò¾úËØ»ùÒò¡£ËûÃÇ·¢ÏÖ£¬ZFNickase´¦Àíϸ°û¿É³É¹¦ÓÃÓÚÌåϸ°ûºËÒÆÖ²£¬Éú³ÉÁË»î²úµÄ»ùÒò°ÐÏòÅ££¨gene-targeted cow£©¡£²¢ÇÒ£¬ÕâЩ»ùÒò°ÐÏòÅ£µÄÈéÖÖзÖÃÚÈÜÆÏÌÑÇò¾úËØ£¬ÌåÍâ·ÖÎö֤ʵÕâÖÖÅ£ÄÌÄܹ»É±ËÀ½ð»ÆÉ«ÆÏÌÑÇò¾ú¡£Ñо¿ÈËÔ±±íʾ£¬ÔÚÕâÒ»²ßÂÔÉÏÈ¡µÃµÄ³É¹¦½«Íƶ¯ÐµÄת»ùÒò¼¼ÊõÈÃũҵºÍÉúÎïҽѧ»ñÒæ¡£ ÉúÎïͨ£ººÎæÍ ÔÎÄÁ´½Ó¼°ÕªÒª£º http://www.nature.com/ncomms/201 ... ull/ncomms3565.html Zinc-finger nickase-mediated insertion of the lysostaphin gene into the beta-casein locus in cloned cows Zinc-finger nickases (ZFNickases) are a type of programmable nuclease that can be engineered from zinc-finger nucleases to induce site-specific single-strand breaks or nicks in genomic DNA, which result in homology-directed repair. Although zinc-finger nuclease-mediated gene disruption has been demonstrated in pigs and cattle, they have not been used to target gene addition into an endogenous gene locus in any large domestic species. Here we show in bovine fetal fibroblasts that targeting ZFNickases to the endogenous ¦Â-casein (CSN2) locus stimulates lysostaphin gene addition by homology-directed repair. We find that ZFNickase-treated cells can be successfully used in somatic cell nuclear transfer, resulting in live-born gene-targeted cows. Furthermore, the gene-targeted cows secrete lysostaphin in their milk and in vitro assays demonstrate the milk¡¯s ability to kill Staphylococcus aureus. Our success with this strategy will facilitate new transgenic technologies beneficial to both agriculture and biomedicine. ×÷Õß¼ò½é£º ÕÅÓ¿ Ñо¿·½Ïò: Îï¿Ë¡Óëת»ùÒò¼¼Êõ¡¢¶¯ÎïÅßÌ¥¹¤³Ì ¸öÈ˼òÀú: 1987Äê7Ô¡ª1987Äê12Ô£¬Î÷±±Å©Òµ´óѧÊÞҽϵÈÎÖú½Ì¡£ 1987Äê12Ô¡ª1990Äê2Ô£¬Î÷±±Å©Òµ´óѧÊÞҽϵ¹¥¶Á²©Ê¿Ñ§Î»¡£ 1990Äê2Ô¡ª1990Äê6Ô£¬Î÷±±Å©Òµ´óѧÊÞҽϵÈν²Ê¦¡£ 1990Äê6Ô¡ª1992Äê12Ô£¬Î÷±±Å©Òµ´óѧÊÞҽϵÈθ±½ÌÊÚ¡¢Ë¶µ¼ ¡£ 1993Äê1Ô¡ª1999Äê9Ô£¬Î÷±±Å©Òµ´óѧÊÞҽϵÈνÌÊÚ¡¢²©µ¼¡£ 1999Äê9Ô¡ª2007Äê9Ô£¬Î÷±±Å©ÁֿƼ¼´óѧ¶¯Îï¿Æ¼¼Ñ§ÔºÈνÌÊÚ¡¢²©µ¼¡£ 2007Äê9ÔÂÖÁ½ñ£¬Î÷±±Å©ÁֿƼ¼´óѧ¶¯ÎïҽѧԺ½ÌÊÚ¡¢²©µ¼¡£ ¿ÆÑÐÏîÄ¿£º 1.Á¼ÖÖÅ£ÅßÌ¥ÌåÍâÉú²ú¼¼ÊõÑо¿Ó뿪·¢£¬ÏîÄ¿±àºÅ£º20061666¡£¹ú¼Ê¿Æ¼¼ºÏ×÷ÏîÄ¿£¬2008-2010Ä꣬×ܾ·Ñ73ÍòÔª¡£ÏîÄ¿Ö÷³ÖÈË¡£ 2. Ìåϸ°û¸ßЧ¿Ë¡¼¼ÊõÑо¿£¬863ÖØµã¿ÎÌ⣬2008-2010Ä꣬×ܾ·Ñ950ÍòÔª¡£¿ÎÌâÖ÷³ÖÈË¡£ 3. ¿¹²¡×ª»ùÒòÅ£ÐÂÆ·ÖÖÅàÓý£¬ÏîÄ¿±àºÅ£º2008ZX08007-004¡£ ÈÙÓþ¼°½±Ï ¹ú¼Ò¼¶¡°ÓÐÍ»³ö¹±Ï×ר¼Ò¡±£¬¡°×ö³öÍ»³ö¹±Ï×µÄÖйú²©Ê¿Ñ§Î»»ñµÃÕß¡±£¬¡±¹ú¼Ò¡°°ÙǧÍòÈ˲ʤ³Ì¡±ÈëÑ¡Õß¡£ »ñµÃ²¿Ê¡¼¶¿Æ¼¼½ø²½½±5ÏÖйúÇàÄê¿Æ¼¼½±¡¢»ôÓ¢¶«ÇàÄê½Ìʦ½±¡¢ÖлªÅ©Òµ¿Æ½Ì½±Å©Òµ¿ÆÑн±¸÷Ò»Ïî¡£ Éç»á¼æÖ°£º ¼æÈιúÎñԺѧλίԱ»áѧ¿ÆÆÀÒé×é³ÉÔ±£¬ÉÂÎ÷ʡѧλίԱ»áίԱ¡¢ÉÂÎ÷Ê¡Õþ¸®¾ö²ß×ÉѯίԱ»áÌØÑûίԱ¡¢ÉÂÎ÷Ê¡ÕþÐίԱ£¬ÉÂÎ÷Ê¡¶¯Îïѧ»á¸±Àíʳ¤£¬ÉÂÎ÷Ê¡ÐóÄÁÊÞҽѧ»á¸±Àíʳ¤¡£ [ Last edited by victorlau on 2013-10-18 at 18:39 ] |
» ±¾ÌûÒÑ»ñµÃµÄºì»¨£¨×îÐÂ10¶ä£©
» ²ÂÄãϲ»¶
288Çóµ÷¼Á Ò»Ö¾Ô¸¹þ¹¤´ó ²ÄÁÏÓ뻯¹¤
ÒѾÓÐ16È˻ظ´
0805Çóµ÷¼Á
ÒѾÓÐ4È˻ظ´
Çóµ÷¼Á£¬Ò»Ö¾Ô¸±±ÁÖʳƷÓëÓªÑø095500£¬301·Ö£¬ÒѹýÁù¼¶£¬ÓпÆÑоÀú
ÒѾÓÐ3È˻ظ´
0856²ÄÁÏÓ뻯¹¤µ÷¼Á£¬339
ÒѾÓÐ5È˻ظ´
311£¨085601£©Çóµ÷¼Á
ÒѾÓÐ10È˻ظ´
080200רҵ277·Ö£¬Çó´ø×ߣ¡
ÒѾÓÐ4È˻ظ´
359Çóµ÷¼Á
ÒѾÓÐ6È˻ظ´
342Çóµ÷¼Á
ÒѾÓÐ6È˻ظ´
070300»¯Ñ§279Çóµ÷¼Á
ÒѾÓÐ10È˻ظ´
086000ÉúÎïÓëÒ½Ò©298µ÷¼ÁÇóÖú
ÒѾÓÐ5È˻ظ´
» ÇÀ½ð±ÒÀ²£¡»ØÌû¾Í¿ÉÒԵõ½:
ɽ¶«½¨Öþ´óѧ²ÄÁÏÀàÑо¿ÉúÕÐÉúµ÷¼Á
+3/173
Çൺ´óѧ¹¦ÄÜ·Ä֯ƷÓëÏȽø²ÄÁÏÑо¿Ôº2026˶ʿÑо¿ÉúÕÐÉúµ÷¼Á
+1/89
º¸½ÓרҵӦ½ì±ÏÒµÉúÕÐÆ¸
+1/81
ɽ¶«Çൺ-µ¼Ê¦ÕÐÉú
+1/52
2026 Äêɽ¶«½¨Öþ´óѧ½ðÊôÔö²ÄÖÆÔì·½Ïò˶ʿÑо¿Éúµ÷¼Á¹«¸æ
+1/38
н®Ì¼ÄÉÃײÄÁÏÖÆ±¸ÓëÓ¦ÓÃÖØµãʵÑéÊÒ2026ÄêÕÐÆ¸ÆôÊÂ
+1/38
2026ÄêÎ人·ÄÖ¯´óѧ²ÄÁÏѧԺµË²¨½ÌÊÚÍŶӽÓÊÕµ÷¼Á£¨Ë¶Ê¿Éú4Ãû£©
+1/38
¹þ¶û±õ¹¤Òµ´óѧÉîÛÚ ½ðÊôÔö²ÄÖÆÔì·½Ïò²©Ê¿ÕÐÉú
+1/33
Î÷ÄϿƼ¼´óѧÑîÎıò½ÌÊÚ¿ÎÌâ×é½ÓÊÕ²ÄÁÏ¡¢»¯¹¤¡¢»¯Ñ§ÀàרҵÑо¿Éúµ÷¼Á ¡£
+1/18
ÑĮ̀´óѧ»·¾³Óë²ÄÁϹ¤³ÌѧԺÏȽø´¢ÄܲÄÁÏÓëÆ÷¼þ¿ÎÌâ×éÕÐÊÕ²ÄÁÏ·½Ïò˶ʿ
+2/14
¡¾ÉϺ£µ÷¼Á¼±Â¼¡¿´úÂë1007/1055/0806/0703./0780./0836µÈËٵ㣡£¡ÉîÔì×ÊÔ´Âú¸ñ£¡
+1/11
Î÷°²¹¤³Ì´óѧÄÜÔ´»¯¹¤Ð²ÄÁÏÑз¢ÏîÄ¿×é½ÓÊÕ2026Äê¼ÆËã»úÀàµ÷¼ÁÑо¿Éú
+1/9
¡¾²©Ê¿ÕÐÉú¡¿Î人¿Æ¼¼´óѧÕÐÊÕ²ÄÁÏ¡¢»¯¹¤¡¢»·¾³¡¢Ò±½ðÀà2026Äê¡°ÉêÇë-¿¼ºË¡±ÖƲ©Ê¿Éú
+1/8
Ìì½òÀí¹¤´óѧÕÐÊÕ²ÄÁÏ¡¢»¯Ñ§ºÍÎïÀí±³¾°Ë¶Ê¿µ÷¼ÁÉú
+1/8
¹ú¼Ò˫һÁ÷¸ßУ-¹ú¼Ò¼¶ÇàÄêÈ˲ſÎÌâ×鲩ʿÕÐÉú
+1/6
Ñàɽ´óѧÈ˹¤ÖÇÄÜѧԺ£¨Èí¼þѧԺ£©¡°ÖªÊ¶·¢ÏÖÓëÍÆ¼ö¡±ÍŶÓ˶ʿÕÐÉú/µ÷¼Á
+1/5
»¶Ó¹ã´ó¿¼Éúµ÷¼Áµ½Î÷°²¹¤³Ì´óѧ»·¾³Ó뻯ѧ¹¤³ÌѧԺ
+1/4
»·¾³Î¢ÉúÎïÓëÎÛȾ¿ØÖÆÑо¿ËùÕÐÊÕ2026˶ʿÑо¿Éú
+1/3
±±¾©¸ßУ¸±Ð£³¤ÍŶÓÕÐÊÕ»úеÀ࣬»·¾³Ààѧ˶ºÍר˶
+1/1
Ó¢¹úÈ«¶î×ÊÖú²©Ê¿ÕÐÉú£üÉ䯵ÓëÎÞÏßϵͳ·½Ïò£¨È«ÇòÕÐÉú£©
+1/1
5Â¥2013-10-22 08:29:14
2Â¥2013-10-20 17:10:20
6Â¥2013-10-22 11:29:56
7Â¥2013-10-22 12:20:52














»Ø¸´´ËÂ¥
ÎÒÒªÇà´º