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Pichia pastoris is a highly successful system for production of a wide variety of recombinant proteins. Several factors have contributed to its rapid acceptance, the most important of which include: (1) a promoter derived from the alcohol oxidase I (AOX1) gene of P. pastoris that is uniquely suited for the controlled expression of foreign genes; (2) the similarity of techniques needed for the molecular genetic manipulation of P. pastoris to those of Saccharomyces cerevisiae; (3) the strong preference of P. pastoris for respiratory growth, a key physiological trait that greatly facilitates its culturing at high-cell densities relative to fermentative yeasts; and (4) the decision in 1993 by Phillips Petroleum Company in Bartlesville, Okla. (and continued by Research Corporation Technologies [RCT]), to release the P. pastoris expression system to academic research laboratories, the consequence of which has been an explosion in the knowledge base on the system as described in numerous recent publications. |
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