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haolw411

金虫 (小有名气)

[求助] 英文文献翻译

学制剂的,该文献很是看不懂,求大神帮忙翻译文献中的以下两段:
    We have previously reported the critical roles   of Rac2 in a wide variety of primary hematopoietic cells, including the regulation of adhesion,migration, oxidase activity, and gene expression.These same functions have been attributed to Rac1 in nonhematopoietic cells in whichRac2 is not expressed. However, the roles of Rac1 compared with the roles of Rac2 in blood cells, which express both GTPases, remain to be elucidated.
    Because homozygous Rac1-deficient mice die at  E8 (embryonic day 8) in utero , we generated mice with a conditional Racl(flox)allele (Rac1 flox/flax)(fig S1).These were compared with mice that were homozygous for both the Racl flox allele and a Rac2-null allele(Rac1flox/flox;Rac2–/–). Floxed Rac1 sequences were deleted by means of two methods, and the deletion of Rac1 sequences was confirmed with polymerase chain reactions (PCRs) and immuno-blots (figs. S2 and S3).
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shs912vv

银虫 (小有名气)

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haolw411(phu_grassman代发): 金币+5, thanks 2014-01-16 16:01:37
因为rca-1纯合子在子宫内胚胎形成的第八天就死了,所以我们采用了条件等位敲出的Rac-1(flox/flox)小鼠。这些小鼠将于等位敲出Rac-1小鼠和Rac-2敲出小鼠比较。Rac-1序列通过两种方法删除,并且这一结果得到了pcr以及western bolt的验证
3楼2013-10-27 12:11:55
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stingdhk

铁杆木虫 (著名写手)

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haolw411(phu_grassman代发): 金币+10, thanks 2014-01-16 16:01:26
我们之前也报道过Rac2在各种主要的造血细胞中的重要作用,包括粘附,迁移,氧化酶活性以及基因表达。在Rac2未表达的非造血细胞中,相同的功能主要是依赖Rac1的。与血细胞中可表达两种酶的Rac2相比,Rac1的作用仍不十分明确。
2楼2013-10-22 21:36:40
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Titanic416

银虫 (知名作家)

北京方便面真方便

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真强必胜: 交流,非应助请勿点击应助 2014-01-16 11:27:12
楼主你好,推荐使用像谷歌翻译,有道等等翻译工具,然后自己适当的调整一下语序,毕竟还是要有自己的思路的,而且最好自己学会一些翻译的技巧,毕竟以后还有很多论文、文献要看的。1、2楼已经给出了自己的答案,我就不贴答案咯,只谈一下自己的想法,希望对你有用!O(∩_∩)O谢谢~
阳光总在风雨后,请相信有彩虹!
4楼2013-10-27 13:24:35
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