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Comparing the values of the three extraction variants of chlorophyll a, best results were obtained with methanol and acetone.Chlorophyll b extraction with diethyl ether registered low results obtained in all the tested vegetables, being close to the limit of detection of the spectrophotometer. Extraction with methanol resulted in high values of chlorophyll b in peppers and cucumbers; the peppers values ranged between 2.52 mg/100 g and 63.45 mg/100 g fresh product and the cucumbers values ranged from 254.90 mg/100 g to 412.31 g/100 g fresh product. When using acetone, the extraction of chlorophyll b is decreased in tomatoes, near the limit of detection of the device and high in peppers and cucumbers. Results of the extraction of chlorophyll b showed that acetone solvent determined the best results followed by extraction with methanol. Statistical interpretation of the results for chlorophyll a determination showed that the best results were obtained from extraction with methanol where the coefficient of variation was 41.07% in cherry tomatoes, 55.97% in common tomatoes, 51.53% in peppers and 52.15% in cucumbers (Table 2). In the case of chlorophyll b extraction, the best coefficients of variation were obtained using extraction with methanol respectively 13.79% in cherry tomatoes 19.57% in common tomatoes, 66.57% in peppers and 47.95% in cucumbers (Table 2). Diethyl ether solvent determined a different extraction of carotene, so at tomatoes the values ranged between 1.20 mg/100 g and 21.91 mg/100 g in fresh product, while at peppers and cucumbers low extraction values were obtained (Table 1). |
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Comparing the values of the three extraction variants of chlorophyll a, best results were obtained with methanol and acetone.ͨ¹ý±È½ÏÈýÖÖÝÍȡֵ±äÒìµÄÒ¶ÂÌËØ£¬¼×´¼ºÍ±ûͪµÄÌáÈ¡´ïµ½ÁË×îºÃµÄ½á¹û¡£Chlorophyll by extraction with diethyl ether registered low results obtained in all the tested vegetables, being close to the limit of detection of the spectrophotometer.ÓÃÒÒÃÑÌáÈ¡µÄÒ¶ÂÌËØÊÇËùÓвâÊÔµÄÊß²ËÀàÖÐÒ¶ÂÌËØÌáÈ¡Á¿×îµÍµÄ£¬½Ó½ü¼ì²âµÄ·Ö¹â¹â¶È¼ÆµÄ¼«ÏÞÁË¡£ Extraction with methanol resulted in high values of chlorophyll b in peppers and cucumbers; ¼×´¼µÄÌáÈ¡ÖÂʹÇཷºÍ»Æ¹ÏµÄÒ¶ÂÌËØµÄº¬Á¿¼«¸ß¡£the peppers values ranged between 2.52 mg/100 g and 63.45 mg/100 g fresh product and the cucumbers values ranged from 254.90 mg/100 g to 412.31 g/100 g fresh product.ÿһ°Ù¿ËÐÂÏÊÇཷµÄÒ¶ÂÌËØº¬Á¿µÄ²¨¶¯·¶Î§ÊÇ2.25-63.45ºÁ¿Ë£¬Ã¿Ò»°Ù¿ËÐÂÏʻƹϵÄÒ¶ÂÌËØº¬Á¿·¶Î§ÊÇ254.9-412.31ºÁ¿Ë¡£ When using acetone, the extraction of chlorophyll b is decreased in tomatoes, near the limit of detection of the device and high in peppers and cucumbers.¶øÊ¹Óñûͪʱ£¬Î÷ºìÊÁµÄÒ¶ÂÌËØÝÍÈ¡Á¿¾Í¼õÉÙÁË£¬½Ó½ü¼ì²âÉ豸µÄ¼«ÖµÁË£¬µ«ÊÇÇཷºÍ»Æ¹ÏµÄÒ¶ÂÌËØº¬Á¿È˱£³Ö½Ï¸ßˮƽ¡£ Results of the extraction of chlorophyll b showed that acetone solvent determined the best results followed by extraction with methanol.½á¹ûÏÔʾ£¬Ê¹ÓñûͪÈܼÁÀ´ÝÍȡҶÂÌËØÓÅÓÚʹÓü״¼ÝÍÈ¡¡£ Statistical interpretation of the results for chlorophyll a determination showed that the best results were obtained from extraction with methanol where the coefficient of variation was 41.07% in cherry tomatoes, 55.97% in common tomatoes, 51.53% in peppers and 52.15% in cucumbers (Table 2).Ò¶ÂÌËØµÄ¾ö¶¨ÒòËØµÄÊý¾Ý·ÖÎö½á¹û±íÃ÷£¬Óü״¼ÔÚʥŮ¹ûÖÐÝÍÈ¡µÄÒ¶ÂÌËØº¬Á¿ÊÇ×î¸ßµÄ£¬ËüµÄ±äÒìϵÊý¸ß´ï41.07%£¬Æä´ÎÊÇÆÕͨÎ÷ºìÊÁµÄ±äÒìϵÊýÊÇ55.97%£¬ÔÙ´ÎÊÇÇཷ51.53%£¬»Æ¹ÏÊÇ52.15%¡££¨±í2£© In the case of chlorophyll b extraction, the best coefficients of variation were obtained using extraction with methanol respectively 13.79% in cherry tomatoes 19.57% in common tomatoes, 66.57% in peppers and 47.95% in cucumbers (Table 2).ÔÚÒ¶ÂÌËØÝÍÈ¡µÄʵÑéÖУ¬µÃµ½µÄÓü״¼ÝÍÈ¡µÄ×î¼Ñ±äÒìϵÊý·Ö±ðÊÇʥŮ¹ûÖÐ13.79% £¬ÆÕͨÎ÷ºìÊÁÖÐ19.57%£¬ÇཷÖÐ 66.57%£¬»Æ¹ÏÖÐ47.95%¡££¨±í2£© Diethyl ether solvent determined a different extraction of carotene, so at tomatoes the values ranged between 1.20 mg/100 g and 21.91 mg/100 g in fresh product, while at peppers and cucumbers low extraction values were obtained (Table 1).ÒÒ´¼ÈܼÁÓ°ÏìÁ˺úÂܲ·ËصÄÝÍÈ¡Á¿£¬Òò´ËÔÚÿһ°Ù¿ËÐÂÏʵÄÎ÷ºìÊÁÖкúÂܲ·Ëصĺ¬Á¿²¨¶¯·¶Î§ÊÇ1.20-21.91ºÁ¿Ë£¬¶øÇཷºÍ»Æ¹ÏµÄºúÂܲ·ËصÄÝÍÈ¡Á¿¼«µÍ¡££¨±í1£© "coefficient of variation "ÓÉÓÚ´Ë´ÊÔÚ²»Í¬ÁìÓòµÄÒë·¨²»Í¬£¬ÓÉÓÚ±¾ÈËרҵÏÞÖÆ£¬Òò´ËÔÝʱ·ÒëΪ¡°±äÒìϵÊý¡±£¬Çë×÷Õß×ÔÐÐÕå×㬲»Êʵ±ÇëÐ޸ģ¬Ð»Ð»£¡Ò²»¶Ó¹ã´ó°®ºÃ·ÒëµÄÈËÊ¿ÅúÆÀÖ¸Õý£¬Ð»Ð»¡£ÃÀ²©·Òë×£Äú¹úÇì¿ìÀÖ£¡ |
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Â¥ÉϵķÒë²»´í¡£ ÓÐÒ»¸ö¹Ø¼üµØ·½£¬ÔÚ´ËÌá³ö¡£Â¥ÉϰÑchlorophyll a, chlorophyll bͳͳ·Òë³ÉÒ¶ÂÌËØ£¬ÕâÊDz»¶ÔµÄ¡£Ó¦·Ö±ð·Òë³ÉÒ¶ÂÌËØaºÍÒ¶ÂÌËØb£¬ËüÃÇ·Ö±ðÊÇÒ¶ÂÌÌåÉ«ËØµÄÁ½ÖÖÖØÒªÐÎʽ£¬¾ßÓв»Í¬µÄ½á¹¹¡£´ËÍ⻹ÓÐÒ¶ÂÌËØc1£¬c2£¬d£¬fµÈ¡£ ¹ØÓÚÒ¶ÂÌËØ£¬Çë²Î¼ûWikipedia https://en.wikipedia.org/wiki/Chlorophyll¡£ |

3Â¥2013-10-06 16:43:00
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4Â¥2013-10-06 18:19:57
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5Â¥2013-10-06 19:51:48
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