| 查看: 355 | 回复: 1 | ||
卡西西VS鸣人金虫 (小有名气)
|
[求助]
求助文献 已有1人参与
|
|
Use of acetylcholinesterase activity to detect sublethal toxicity in stream invertebrates exposed to low concentrations of organophosphate insecticides Kristin E. Day, Ian M. Scott 文献网站http://www.sciencedirect.com/science/article/pii/0166445X9090021G 接受邮箱：hepin1314@126.com Abstract Acetylcholinesterase (AChE) activity was measured in selected species of aquatic invertebrates using two methodologies based on the Ellman technique — a modified kit procedure using the spectrophotometer (BMC procedure) and a microplate assay. Levels of AChE ranged from 12.7 mU/mg protein for Daphnia magna to 96.9 mU/mg protein for Hydropsyche spp. The coefficients of variation (CVs) for the microplate technique were much lower than the CVs for the BMC procedure (e.g., 6.9 to 25.5% vs. 34.6 to 55.4%) and allowed measurements within single whole organisms or individual head capsules. Exposure of invertebrates to low concentrations of the organophosphate insecticides, azinphosmethyl and fenitrothion, did not result in a significant depression in AChE levels with one exception, i.e. levels of AChE in Hyalella azteca declined to 55.2% of control levels following 24 h exposure to 2.0 μg azinophosmethyl/l. Exposure for longer periods (48 to 96 h) did not result in reduced levels. Exposure of the stonefly, Claassenia sp., to chlorpyrifos significantly lowered AChE levels by 30.7–45.1% at concentrations approaching lethality (⩾40 μg/l). The results indicate that measurement of the activity of AChE in aquatic invertebrates exposed to field concentrations of organophosphate insecticides may be a useful biochemical technique but only for detecting acute toxicity following exposure in the field. In addition, choice of species may be important in the detection of sublethal effects. [ Last edited by 卡西西VS鸣人 on 2013-10-2 at 08:32 ] |
回复此楼» 猜你喜欢
售SCI一区文章，我:8O5.5.1.O5.4,科目全,可伽急
已经有3人回复
-
售SCI一区文章，我:8.O.551.O.5.4,科目全,可伽急
已经有3人回复
-
售SCI一区文章，我:8.O.551.O.5.4,科目全,可伽急
已经有3人回复
-
售SCI一区T0P文章，我:8.O.55.1.O.54,科目齐全,可+急
已经有5人回复
-
售SCI文章，我:8O5.5.1.O.54,科目齐全,可+急
已经有5人回复
-
售SCI一区T0P文章，我:8.O.55.1.O.5.4,科目全,可+急
已经有5人回复
-
售SCI一区T0P文章，我:8.O.55.1.O.5.4,科目全,可+急
已经有3人回复
-
售SCI一区T0P文章，我:8.O55.1.O.54,科目全,可十急
已经有4人回复
-
售SCI一区T0P文章，我:8.O.55.1.O.5.4,科目全,可+急
已经有3人回复
-
售SCI一区文章，我:8.O.551.O.5.4,科目全,可伽急
已经有4人回复
wrs王瑢笙
木虫 (正式写手)
- 应助: 0 (幼儿园)
- 金币: 3328.4
- 红花: 5
- 沙发: 1
- 帖子: 310
- 在线: 42.5小时
- 虫号: 2425415
- 注册: 2013-04-19
- 性别: GG
- 专业: 植物化学保护
【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★
卡西西VS鸣人: 金币+10, ★★★★★最佳答案 2014-01-10 10:34:47
卡西西VS鸣人: 金币+10, ★★★★★最佳答案 2014-01-10 10:34:47
| 你看看是不是这篇文献！希望对你有帮助！ |
» 本帖附件资源列表
-
欢迎监督和反馈：小木虫仅提供交流平台，不对该内容负责。
本内容由用户自主发布，如果其内容涉及到知识产权问题，其责任在于用户本人，如对版权有异议，请联系邮箱：xiaomuchong@tal.com - 附件 1 : Useofacetylcholinesteraseactivitytodetectsublethaltoxicity.pdf
2013-12-20 20:39:42, 748.87 K
2楼2013-12-20 20:39:31
10