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547star

木虫 (著名写手)

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注册: 2005-05-07
专业: 系统生物学

[求助] 求BGSC 的1A976,1P19

BGSCID: 1A976

Taxonomy Description: Bacillus subtilis subsp. subtilis

Gene Descriptor: Em his nprE18 aprE3 eglS(DELTA)102bglT/bglS(DELTA)EVlacA:

xylA-comK

Original Code: SCK6

Shipping Conditions: Filter disks within foil packet

Growth Conditions: LB, Tryptose Blood Agar Base, Nutrient Agar, or other rich medium containing erythromycin (1 µg/ml)

Temperature: 37

References: Zhang XZ, Zhang YH. Simple, fast and high-efficiency transformation system for directed evolution of cellulase in Bacillus subtilis. Microb Biotechnol. 2011 Jan;4(1): 98-105 (PMID: 21255377)
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BGSCID: 1P19

Taxonomy Description: Bacillusphage

Gene Descriptor: phi29

Original Code: phi29

Shipping Conditions: Lysate within screw-top vial

Growth Conditions: To amplify after reception, streak host confluently on LB agar plate and immediately drop ~10 µl of lysate on the lawn. Incubate at 30°C overnight.Cut out lysed area with surrounding bacteria and transfer to flask with 20 ml LB broth.Shake at 30°C at

Temperature: 37

References: Reilly BE, Spizizen J. Bacteriophage Deoxyribonucleate Infection of Competent Bacillus subtilis. J Bacteriol. 1965 Mar;89: 782-90 (PMID: 14273661)Camacho A, Salas M. DNA bending and looping in the transcriptional control of bacteriophage phi29. FEMS Microbiol Rev. 2010 Sep;34(5): 828-41. doi: 10.1111/j.1574-6976.2010.00219.x. Epub 2010 Mar 17 (PMID: 20412311)
有计划从BGSC卖菌株的兄弟姐妹，如果能为我代购1A976和1P19，请联系我。半年内有效。

[ Last edited by 547star on 2013-9-5 at 21:05 ]

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