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[ÇóÖú] Streptococcus equi SED 9 ÊÇʲô¾úÖÖ

ÎÄÏ×£ºOPTIMIZATION OF SOME PHYSICAL AND NUTRITIONAL PARAMETERS
FOR THE PRODUCTION OF HYALURONIDASE
BY STREPTOCOCCUS EQUI SED 9
×÷Õß SABUJ SAHOO*, PRASANA K. PANDA, SATYA R. MISHRA, ANINDITA NAYAK, POLURI

ÎÄÕÂÖÐÏà¹ØÄÚÈÝ£ºA pathological isolate from Orissa University
of Agriculture and Technology, Bhubaneswar,
Orissa identified as Streptococcus equi SED 9 producing
an extracellular hyaluronidase enzyme within
48 h was used for subsequent experiments.

Abstract: The effects of some physical and nutritional parameters were studied for the optimum production of
an extracellular enzyme hyaluronidase employing Streptococcus equi SED 9 by submerged fermentation. The
effects of initial pH, incubation temperature and time, inoculum level and age of inoculum were studied. The
maximum enzymatic activity was obtained with an initial pH 5.5, incubation temperature 37OC, incubation time
for 48 h and inoculum level 10% with inoculum age 48 h. The effects of various carbon and inorganic nitrogen
sources, vitamins, amino acids and growth hormones were studied. The results indicated that dextrose, ammonium
sulfate, nicotinic acid, L-cysteine and kinetin showed the highest enzymatic activity among them.

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