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[资源] Single Molecule Detection in Solution.pdf

Single-Molecule Detection in Solution Methods and Applications
By

Publisher:  Wiley-VCH
Number Of Pages:  400
Publication Date:  2002-01-15
ISBN / ASIN:  3527403108
EAN:  9783527403103

Publisher:  Wiley-VCH
Number Of Pages:  400
Publication Date:  2002-01-15
Sales Rank:  1762785
ISBN / ASIN:  3527403108

Book Description:

The detection of single molecules opens up new horizons in analytical chemistry, biology and medicine. This discipline, which belongs to the expanding field of nanoscience, has been rapidly emerging over the last ten years.
This handbook provides a thorough overview of the field. It begins with basics of single molecule detection in solution, describes methods and devices (fluorescense correlation spectroscopy, surface enhanced Raman scattering, sensors, especially dyes, screening techniques, especially confocal laser scanning microscopy). In the second part, various applications in life sciences and medicine provide the latest research results.
This modern handbook is a highly accessible reference for a broad community from advanced researchers, specialists and company professionals in physics, spectroscopy, biotechnology, analytical chemistry, and medicine. Written by leading authorities in the field, it is timely and fills a gap - up to now there exists no handbook concerning this theme.

Table of Contents

Obituary
List of Contributors
1 Single Molecule Detection in Liquids and on Surfaces under Ambient Conditions: Introduction and Historical Overview 1
2 Theoretical Foundations of Single Molecule Detection in Solution 21
3 Conceptual Basis of Fluorescence Correlation Spectroscopy and Related Techniques as Tools in Bioscience 69
4 Surface-enhanced Raman Scattering (SERS) - A Tool for Single Molecule Detection in Solution 121
5 Single Molecule Detection on Surfaces with the Confocal Laser Scanning Microscope 145
6 Spectroscopy of Individual Photosynthetic Pigment-Protein Complexes 185
7 Single Dye Tracing for Ultrasensitive Microscopy on Living Cells 231
8 Single Molecule Identification in Solution: Principles and Applications 247
9 Studying Molecular Motors on the Single Molecule Level 273
10 The Chemistry of a Single Enzyme Molecule 293
11 Single Molecule Detection of Specific Nucleic Acid Sequences 303
12 Single Molecule Detection in the Near-infrared 323
Name Index 363
Subject Index 365
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