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我有一篇文章做的也是基因的克隆和组织差异表达荧光定量PCR，Four trypsin-like serine proteases, designated as Fctry1 , Fctry2 , Fctry3 , and Fctry4 , were obtained from the hepatopancreas of the Chinese shrimp, Fenneropenaeus chinensis . Three trypsin-lik e serine proteases,specifi cally Fctry1 , 2 , and 3 were observed to have the conserved catalytic triad (H, D, and S). Regarding Fctry4 , the catalytic triad S was substituted by F, and the mature peptide was found to be inactive. Further analysis for similarities indicated that Fctry1 , 2 and 4 were 89–92% identical to trypsins from Pacific white shrimp (Litopenaeus vannamei); while Fctry3 was only 42% identical to trypsin s from salmon louse ( Lepeophtheirus salmonis ). The genomic organizations of Fctry1 , 2 and 4 are also quite different from Fctry3 .So, Fctry3 may be a new member of the trypsin-like serine protease family. Moreover, a semi-quantitative reverse transcription polymerase chain reaction (PCR) and quantitative real-time PCR was carried out to analyze the distribution pro fi les and exp ression patterns af ter they were subjected to a bacterial and viral challen ge. The results sh owed that the four trypsin -like serin e proteases were upregulated in the hepatopancreas of shrimp infected with the white spot syndrome virus (WSSV), andFctry3 increased after a bacteria challenge. Therefore, these trypsin-like serin e proteases might be involved in the innate defense reactions against different pathogens in Chinese shrimp.就是这种模式，不过我只做了一个基因。有人说现在这种文章发在Fish shellfish immunology很难了，让我改投到1.几我又舍不得，请问各位高手这种文章投到Fish shellfish immunology有机会接收不？ |
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三甲基碘化亚砜的氧化反应
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