| ²é¿´: 745 | »Ø¸´: 2 | |||
| ±¾Ìû²úÉú 1 ¸ö ·ÒëEPI £¬µã»÷ÕâÀï½øÐв鿴 | |||
| µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû | |||
huojinlong8610½ð³æ (СÓÐÃûÆø)
|
[ÇóÖú]
ÈóɫӢÓï
|
||
|
ÒªÇóÈóɫӢÓï ¡¾ÕªÒª¡¿Ä¿µÄ »ñµÃ°æÄÉ΢ÐÍÖí½ü½»ÏµÉú³¤¼¤ËØÊÜÌ壨Growth hormone receptor£¬GHR£©»ùÒòÐòÁУ¬Í¨¹ýÉúÎïÐÅϢѧ·ÖÎöÔ¤²âGHR¹¦Äܲ¢½øÐÐGHR mRNA¶à×éÖ¯±í´ïÆ×·ÖÎö¡£·½·¨ ÒÔ°æÄÉ΢ÐÍÖí½ü½»ÏµµÄ¸ÎÔà×é֯Ϊ²ÄÁÏÌáÈ¡RNA£¬RT-PCR·½·¨À©ÔöGHR»ùÒò±àÂëÇøÐòÁУ¬½«ÐòÁÐÁ¬½ÓÖÁpMD18-TÔØÌå½øÐпË¡¡¢²âÐòºÍÉúÎïÐÅϢѧ·ÖÎö£»°ë¶¨Á¿·½·¨¼ì²âGHR mRNAÔÚBMI²»Í¬×éÖ¯Öбí´ïÁ¿µÄ²îÒì¡£½á¹û ³É¹¦¿Ë¡ÁËBMI GHR ±àÂëÇøÐòÁУ¬Ìá½»GenBank»ñµÃµÇ¼ºÅKC999114¡£¸Ã»ùÒòCDS³¤1917 bp£¬±àÂë638¸ö°±»ùËá¡£ÉúÎïÐÅϢѧ·ÖÎö±íÃ÷£¬Ó볤°×ÖíµÄGHRÐòÁÐÏà±ÈBMI´æÔÚ4´¦°±»ùËáÌæ»»£¬·Ö±ðΪp. E381D£¬p. A409S£¬p. L556VºÍp. A580G£¬¾ù·¢ÉúÔÚGHBPÇø¡£GHR»ùÒò¶à×éÖ¯±í´ïÆ×·ÖÎöÏÔʾ£ºGHR mRNA¼¸ºõÔÚ¸÷×éÖ¯ÖоùÓбí´ï£¬ÆäÖÐÔÚÉñ¾ÏËά¡¢ÐÄ¡¢¸Î¡¢Æ¢¡¢·Î¡¢Ð¡³¦¡¢Âѳ²ºÍ¼¡ÈâÖбí´ïÁ¿¶¼ºÜ¸ß£¬ÔÚ´óÄÔ¡¢Éö¡¢Î¸ºÍÒÈÖеıí´ïÁ¿½ÏµÍ¡£½áÂÛ GHRµÄ°±»ùËáÌæ»»¿ÉÄÜ»áÓ°ÏìGHRÓëGHµÄ½áºÏ£¬µ¼ÖÂÆäÌåÐͰ«Ð¡¡£ [Abstract] Objective To get the BMI GHR gene sequence, predict its function by bioinformatics analysis and obtain the GHR mRNA tissues transcription profile. Methods BMI GHR cDNA sequence was cloned from liver RNA by RT-PCR. Inserted the product into pMD18-T vector for clone, sequencing and bioinformatics analysis. Conducted semi-quantitative RT-PCR to determine its expression in different tissues. Results The GHR cDNA sequence was cloned and got the GenBank Accession No. KC999114. The encoding sequence was 1917 bp and encoded a protein of 638 amino acids. Bioinformatics analysis showed that there were four amino acids substitutions were found in the GHR binding protein superfamilies between BMI and Landrace pig GHR protein. The substitutions were p. E381D, p. A409S, p. L556V and p. A580G. GHR mRNA tissues expression analysis revealed that GHR mRNA was expressed in almost all tissues. It was most highly expressed in the nerve fiber, heart, liver, spleen, lung, small intestine, ovary and muscle while weakly expressed in the brain, kidney, stomach and pancreas. Conclusions The BMI GHR substitutions maybe weaken the interaction of GHR and GH which can lead to BMI dwarfism. |
» ²ÂÄãϲ»¶
Èç¹û´Ë¿ÌÄãÕýÔÚΪ¹ú»ù¸Ðµ½½¹ÂÇ£¬²»·ÁÀ´ÌýÌýÕâÊס¶»ù½ðÖ®Íâ¡·
ÒѾÓÐ3È˻ظ´
½ñÈÕ²»·Å°ñ£¿Íø´«¹ú×ÔȻԤ¼Æ 8 Ô 27 Èտɲé½á¹û
ÒѾÓÐ21È˻ظ´
ÄÜ·ñÍ˳ö²ÎÓëµÄÃæÉÏÏîÄ¿½â³ýÏÞÏî
ÒѾÓÐ17È˻ظ´
¹À¼ÆÊÇÖÜËÄ
ÒѾÓÐ4È˻ظ´
ÎÒÃæÉÏÍêµ°ÁË
ÒѾÓÐ9È˻ظ´
2026¹ú×ÔÈ»º¯ÆÀ·Ñµ½ÕË
ÒѾÓÐ18È˻ظ´
½¨Òé»ù½ð·¢²¼Ìáǰ¸ø³öÃ÷È·µÄʱ¼äµã
ÒѾÓÐ15È˻ظ´
·¶½øÖоÙÒ»ÎĵÄÖÐÐÄ˼Ïë
ÒѾÓÐ7È˻ظ´
ÅóÓÑȦ¿´µ½µÄ
ÒѾÓÐ9È˻ظ´
ÈÃÎÒÖÐÒ»¸öÃæÉϰɣ¡
ÒѾÓÐ16È˻ظ´
lemon_time
½ð³æ (СÓÐÃûÆø)
- ·ÒëEPI: 1
- Ó¦Öú: 0 (Ó×¶ùÔ°)
- ½ð±Ò: 784.5
- Ìû×Ó: 55
- ÔÚÏß: 13.8Сʱ
- ³æºÅ: 1337133
- ×¢²á: 2011-07-04
- רҵ: ¶¯Îï×ÊÔ´Óë±£»¤
¡¾´ð°¸¡¿Ó¦Öú»ØÌû
¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ...
huojinlong8610: ½ð±Ò+80, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸ 2013-07-29 17:46:40
huojinlong8610: ½ð±Ò+80, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸ 2013-07-29 17:46:40
| [Abstract] Objective To get the sequence of BMI GHR gene, predict its function by bioinformatics analysis and investigate its mRNA expression profile in different tissues. Methods BMI GHR cDNA sequence was cloned from liver RNA by RT-PCR. Then the products were inserted into pMD 18-T vector for cloning, sequencing and bioinformatics analysis. It was to determine GHR mRNA expression in different tissues by conducting semi-quantitative RT-PCR. Results The GHR cDNA sequence was cloned and the the GenBank Accession No. was KC999114. The encoding sequence was 1917 bp and encoded a protein of 638 amino acids. The bioinformatics analysis result showed that there were four amino acids substitutions at the GHBP superfamilies of GHR protein comparing BMI with Landrace. The substitutions were p. E381D, p. A409S, p. L556V and p. A580G. Tissues expression analysis revealed that GHR mRNA was expressed in almost all tissues. It was most highly expressed in the nerve fiber, heart, liver, spleen, lung, small intestine, ovary and muscle while weakly expressed in the brain, kidney, stomach and pancreas. Conclusions The GHR substitutions may affect the interaction of GHR and GH which can infulence the BMI growth and development. |
3Â¥2013-07-29 17:44:51
biomed_fanyi 
гæ (³õÈëÎÄ̳)
¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï
huojinlong8610: ½ð±Ò+20, ·ÒëEPI+1, ¡ïÓаïÖú 2013-07-29 17:46:31
huojinlong8610: ½ð±Ò+20, ·ÒëEPI+1, ¡ïÓаïÖú 2013-07-29 17:46:31
|
±¾ÌûÄÚÈݱ»ÆÁ±Î |
2Â¥2013-07-29 08:31:08









»Ø¸´´ËÂ¥
20