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lili870818

新虫 (初入文坛)

[求助] 求助为什么跑双向电泳一维时的时候电压上不去

求各位大神帮忙,小女子是电泳新手今天刚刚做完,跑的太不理想了。样品是大豆球蛋白,我用nagano发方法制的后来对其改性得固体粉末。将粉末用TCA/丙酮处理,后在用丙酮洗3次,然后进行一维电泳,样品制备液和水化液都是根据GE的标书配置。一维电泳条件:30V 12h(水化),200V 1h,500V 1h,grd 8000V 3h,8000V 80000hrv,1000V 10h。但是跑到8000V 80000hrv 电压就上不去了,能达到3580V左右。请求帮忙,如何能使电压上去?
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daibingling

铁虫 (正式写手)

【答案】应助回帖

是不是蛋白里的杂质太多了?
3楼2013-12-04 14:34:31
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lidejin

新虫 (初入文坛)

你的两性电解质用多少
2楼2013-12-04 11:21:38
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