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[资源] ECACC 的细胞培养计数指南--Fundamental Techniques in Cell Culture

Fundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition
In response to popular demand the European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich have produced a 2nd Edition of ‘Fundamental Techniques in Cell Culture’. This compact laboratory handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation and quality control. Additionally a series of 10 detailed protocols are provided, which are routinely used in the ECACC laboratories.

Chapters       Title
1       Introduction
2       Design and Equipment for the Cell Culture Laboratory
3       Safety Aspects of Cell Culture
4       Sourcing of Cell Lines
5       Cell Types & Culture Characteristics
6       The Cell Environment (including types of culture medium)
7       Cryopreservation and Storage of Cells
8       Good Cell Banking Practices
9       Quality Control Considerations in Cell Culture
10       Authentication of Cell Lines
11       Alternative Culture Systems
12       Basic Techniques - The “Do’s and Don’ts” of Cell Culture

Protocols       Title
1       Aseptic Technique and Good Cell Culture Practice
2       Resuscitation of Frozen Cell Lines
3       Subculture of Adherent Cell Lines
4       Subculture of Semi-Adherent Cell Lines
5       Subculture of Suspension Cell Lines
6       Cell Quantification
7       Cryopreservation of Cell Lines
8       Testing for Bacteria and Fungi in Cell Cultures
9       Testing for Mycoplasma by Culture Isolation
10       Testing for Mycoplasma by Indirect DNA Stain

Glossary of Terms

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附件 1 : Fundamental_Techniques_and_Protocols_for_ECACC_Cell_Lines.pdf

2013-07-27 11:07:28, 2.61 M