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[资源] ECACC 的细胞培养计数指南--Fundamental Techniques in Cell Culture


Fundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition
In response to popular demand the European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich have produced a 2nd Edition of ‘Fundamental Techniques in Cell Culture’. This compact laboratory handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation and quality control. Additionally a series of 10 detailed protocols are provided, which are routinely used in the ECACC laboratories.

Chapters         Title
1         Introduction
2         Design and Equipment for the Cell Culture Laboratory
3         Safety Aspects of Cell Culture
4         Sourcing of Cell Lines
5         Cell Types & Culture Characteristics
6         The Cell Environment (including types of culture medium)
7         Cryopreservation and Storage of Cells
8         Good Cell Banking Practices
9         Quality Control Considerations in Cell Culture
10         Authentication of Cell Lines
11         Alternative Culture Systems
12         Basic Techniques - The “Do’s and Don’ts” of Cell Culture

Protocols         Title
1         Aseptic Technique and Good Cell Culture Practice
2         Resuscitation of Frozen Cell Lines
3         Subculture of Adherent Cell Lines
4         Subculture of Semi-Adherent Cell Lines
5         Subculture of Suspension Cell Lines
6         Cell Quantification
7         Cryopreservation of Cell Lines
8         Testing for Bacteria and Fungi in Cell Cultures
9         Testing for Mycoplasma by Culture Isolation
10         Testing for Mycoplasma by Indirect DNA Stain

Glossary of Terms
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重复下载了一次,可惜了。
不过还是赞一个
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