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ECACC 的细胞培养计数指南--Fundamental Techniques in Cell Culture
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 Fundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition In response to popular demand the European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich have produced a 2nd Edition of ‘Fundamental Techniques in Cell Culture’. This compact laboratory handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation and quality control. Additionally a series of 10 detailed protocols are provided, which are routinely used in the ECACC laboratories. Chapters Title 1 Introduction 2 Design and Equipment for the Cell Culture Laboratory 3 Safety Aspects of Cell Culture 4 Sourcing of Cell Lines 5 Cell Types & Culture Characteristics 6 The Cell Environment (including types of culture medium) 7 Cryopreservation and Storage of Cells 8 Good Cell Banking Practices 9 Quality Control Considerations in Cell Culture 10 Authentication of Cell Lines 11 Alternative Culture Systems 12 Basic Techniques - The “Do’s and Don’ts” of Cell Culture Protocols Title 1 Aseptic Technique and Good Cell Culture Practice 2 Resuscitation of Frozen Cell Lines 3 Subculture of Adherent Cell Lines 4 Subculture of Semi-Adherent Cell Lines 5 Subculture of Suspension Cell Lines 6 Cell Quantification 7 Cryopreservation of Cell Lines 8 Testing for Bacteria and Fungi in Cell Cultures 9 Testing for Mycoplasma by Culture Isolation 10 Testing for Mycoplasma by Indirect DNA Stain Glossary of Terms |
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2013-07-27 11:07:28, 2.61 M
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