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[求助]
在荧光显微镜下观察GFP的发光，如何制片，抗淬灭剂怎么加？
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| 在荧光显微镜下观察GFP的发光，如何制片，抗淬灭剂怎么加？ |
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 蛋白质生物学实验经验 |
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- 【求助/交流】脂质体转染后单克隆荧光显微镜下看到GFP表达较弱
已经有3人回复
2楼2013-07-12 00:37:13
3楼2013-07-12 10:06:37
凌波丽
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【答案】应助回帖
★ ★
kx444555: 金币+2, 鼓励交流 2013-09-06 13:10:18
kx444555: 金币+2, 鼓励交流 2013-09-06 13:10:18
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MMB 183-green fluorescent protein,绿色荧光蛋白质的实验技术 http://muchong.com/bbs/viewthread.php?tid=5995152 该书没有目录，比较麻烦，我查了一下，楼主可以参见该书P163-P180，chapter-12.Light-Induced Nuclear Targeting of PhytochromeB–sGreen Fluorescent Protein in Plants。 |
4楼2013-07-17 01:47:04
凌波丽
专家顾问 (知名作家)
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专家经验: +218 - MolEPI: 44
- 应助: 397 (硕士)
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- 专业: 生物大分子结构与功能
- 管辖: 生物科学综合
★ ★ ★ ★ ★
kx444555: 金币+5, 鼓励交流 2013-09-06 13:10:30
kx444555: 金币+5, 鼓励交流 2013-09-06 13:10:30
|
MMB 183-green fluorescent protein,绿色荧光蛋白质的实验技术 http://muchong.com/bbs/viewthread.php?tid=5995152 我刚做了MMB 183-green fluorescent protein的一份完整的目录，这样看起来就清楚多了，Word版本在上贴的附件里。帖子里也直接贴上去了。 这里也贴出来吧。 MMB 183-green fluorescent protein Contents 1. Amplification of Representative cDNA Samples from Microscopic Amounts of Invertebrate Tissueto Search for New Genes…………………………………………………………………………………………………..1 2. Use of cobA and cysGA as Red Fluorescent Indicates…………………………………………………………19 3. Circular Permutation of the Green Fluorescent Protein………………………………………………………….31 4. Evolutionary Design of Generic Green Fluorescent Protein Biosensors…………………………………..49 5. Random Insertion of Green Fluorescent Protein into the Regulatory Subunit of Cyclic Adenosine Monophosphate-Dependent Protein Kinase…………………………………………………………57 6. Circular mRNA Encoding for Monomeric and Polymeric Green Fluorescent Protein……………….69 7. Fluorescence Lifetime Imaging (FLlM) of Green Fluorescent Fusion Proteins in Living Cells……89 8. Fluorescence Resonance Energy Transfer (FRET) Applications Using Green Fluorescent Protein…………………………………………………………………………………………………………………………………101 9. Bioluminescence Resonance Energy Transfer Assays for Protein–Protein Interactionsin Living Cells……………………………………………………………………………………………………………………………………..121 10. Whole-Body Fluorescence Imaging with Green Fluorescence Protein…………………………………135 11. Drug-Induced Translocation of Nucleolar Proteins Fused to Green Fluorescent Protein……..151 12. Light-Induced Nuclear Targetingof PhytochromeB–sGreen FluorescentProtein in Plants…………………………………………………………………………………………163 13. Mechanisms of Protein Trafficking……………………………………………………………………………………..171 14. Analysis of Nucleocytoplasmic Transport Using Green Fluorescent Protein……………………….181 15. Transgenic Bovine Embryo Selection Using Green Fluorescent Protein……………………………….201 16. Development of Glycosylphosphatidylinositol-Anchored Enhanced Green Fluorescent Protein…………………………………………………………………………………………………………………………………215 17. Transgenic Zebrafish Expressing Green Fluorescent Protein……………………………………………….225 18. Transgenic Insects………………………………………………………………………………………………………………235 19. Green Fluorescent Protein in Transgenic Plants………………………………………………………………….245 20. Green Fluorescent Protein Calcium Biosensors…………………………………………………………………..255 21. Green Fluorescent Protein Fluobody Immunosensors………………………………………………………..265 22. Green Fluorescent Protein-Based Protein Kinase Biosensor Substrates………………………………275 23. Green Fluorescent Protein Urea Sensors…………………………………………………………………………….287 24. Using Green Fluorescent Protein to Monitor Measles Virus Cell-to-Cell Spreadby Time-Lapse Confocal Microscopy…………………………………………………………………………………………………………….297 25. Tracking and Selection of Retrovirally Transduced Murine Bone Marrow Cells Using Green Fluorescent Protein…………………………………………………………………………………………………………….309 26. Green Fluorescent Protein as a Reporter of Adenovirus-Mediated Gene Transfer and Expression in the Hypothalamic–Neurohypophyseal System………………………………………………..321 27. Enhancement of Green Fluorescent Protein Expression in Adeno-Associated Virus with the Woodchuck Hepatitis Virus Post-Transcriptional Regulatory Element…………………………………331 28. Construction of Infectious Simian Varicella Virus Expressing Green Fluorescent Protein…….341 29. Green Fluorescent Protein in Retroviral VectorConstructs as Marker and Reporter of Gene Expression for Cell and Gene Therapy Applications……………………………………………………………353 |
5楼2013-07-17 02:28:38
6楼2013-09-05 16:11:12
凌波丽
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专家经验: +218 - MolEPI: 44
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7楼2013-09-06 00:29:29
凌波丽
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专家经验: +218 - MolEPI: 44
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- 管辖: 生物科学综合
8楼2013-09-06 00:31:35