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| Open the chest cavity of the mouse to expose the heart, which should still be beating. Carefully insert a 1 mL syringe with attached 27 G needle into the left ventricle and inject fluorescein-conjugated L. esculentum (Tomato) lectin at 100 ug/mouse. Allow 2 min for circulation. Then, perfuse with 1% PFA. Tumor vessel perfusion can now be visualized using fl uorescence microscopy. |
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fuboluben
Ìú¸Ëľ³æ (СÓÐÃûÆø)
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- Ìû×Ó: 143
- ÔÚÏß: 143.9Сʱ
- ³æºÅ: 1148022
- ×¢²á: 2010-11-16
- רҵ: ΢ÉúÎïÒ©Îï
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yin198716: ½ð±Ò+5, ·ÒëEPI+1 2013-07-01 11:20:50
yin198716: ½ð±Ò+5, ·ÒëEPI+1 2013-07-01 11:20:50
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fuboluben
Ìú¸Ëľ³æ (СÓÐÃûÆø)
- ·ÒëEPI: 51
- Ó¦Öú: 2 (Ó×¶ùÔ°)
- ½ð±Ò: 5949.4
- É¢½ð: 8
- ºì»¨: 2
- Ìû×Ó: 143
- ÔÚÏß: 143.9Сʱ
- ³æºÅ: 1148022
- ×¢²á: 2010-11-16
- רҵ: ΢ÉúÎïÒ©Îï

3Â¥2013-07-01 09:44:57














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