| 查看: 538 | 回复: 1 | ||
| 本帖产生 1 个 翻译EPI ，点击这里进行查看 | ||
[求助]
论文摘要翻译
|
||
| In this paper, a direct and simple detection method based on surface-enhanced Raman scattering (SERS) named “heat-induced SERS sensing method” is proposed for rapid determination of glutathione in aqueous solutions. It was found that highly enhanced SERS spectra of glutathione can be obtained if the silver colloids adsorbed with the analyte were heated up before the SERS measurement. Besides, it was revealed that silver particles with a size of 60 nm are suitable for this study and that the SERS intensity is also influenced by the dropped sample volume, drying temperature, buffer concentration, and pH of the solution. It is noted that the thiol group of glutathione has a particularly strong interaction with a silver surface compared with other small biological molecules without a thiol group, validating this method to detect glutathione selectively. Under the optimal conditions, the detection of glutathione can be finished within 5 min, and the detection limit of ca. 50 nM can be reached, which is much better than the reported detection limit of glutathione (1 μM) by SERS. The enhancement factor of the proposed heat-induced SERS sensing method for the detection of glutathione is about 7.5 × 106. The proposed method holds a specific selectivity toward glutathione, facilitating its rapid detection in practical applications. |
回复此楼» 猜你喜欢
生物学学硕求调剂：351分一志愿南京师范大学生物学专业
已经有5人回复
-
求调剂，一志愿厦门大学，生物与医药，总分272，本科211
已经有5人回复
-
调剂
已经有9人回复
-
070300化学学硕311分求调剂
已经有11人回复
-
一志愿北交大材料工程总分358求调剂
已经有7人回复
-
308求调剂
已经有12人回复
-
0703化学
已经有18人回复
-
生物学308分求调剂（一志愿华东师大）
已经有5人回复
-
292求调剂
已经有4人回复
-
286求调剂
已经有10人回复
luodiheng
新虫 (小有名气)
- 翻译EPI: 4
- 应助: 0 (幼儿园)
- 金币: 1741.9
- 红花: 1
- 帖子: 155
- 在线: 71.5小时
- 虫号: 264603
- 注册: 2006-07-08
- 性别: GG
- 专业: 有机合成
【答案】应助回帖
★ ★ ★ ★ ★
847037274(phu_grassman代发): 金币+5, 翻译EPI+1, thanks，SERS是表面增强拉曼 2013-11-07 10:19:02
847037274(phu_grassman代发): 金币+5, 翻译EPI+1, thanks，SERS是表面增强拉曼 2013-11-07 10:19:02
| 在此文中，一个基于表面增效Raman扫描（SERS）叫做"热诱导SERS感应手段"的直接而简单的检测手段被提出来用于快速检测水溶液中的谷胱甘肽。如果吸附了被检物的含银胶质物在SERS检测前加热的话，可以得到极大提高的谷胱甘肽SERS光谱。另外还发现，60nm的银粒子是最适合的，另外SERS强度也受样本体积，干燥温度，缓冲液浓度和溶液PH值的影响。另外观察到的是，与其他没有ＳＨ的生物小分子相比，谷胱甘肽的ＳＨ与银表面有着特别强的相互作用，使得这种检测手段具有谷胱甘肽选择性。在最优条件下，谷胱甘肽检测可以在５分钟内完成，检测极限达50 nM，这比报道的谷胱甘肽SERS检测极限1uM好得多。热诱导SERS感应手段相对于普通的谷胱甘肽检测灵敏度提高因子大约能达7.5X106。由于此检测手段对于谷胱甘肽的特异选择性，使得他在实际应用中成为快速检测谷胱甘肽一种手段。 |
2楼2013-06-16 14:56:48
