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Contents

1. Amplification of Representative cDNA Samples from Microscopic Amounts of Invertebrate Tissueto Search for New Genes¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­..1
2. Use of cobA  and  cysGA as Red Fluorescent Indicates¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­19
3. Circular Permutation of the Green Fluorescent Protein¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­.31
4. Evolutionary Design of Generic Green Fluorescent Protein Biosensors¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­..49
5. Random Insertion of Green Fluorescent Protein into the Regulatory Subunit of Cyclic Adenosine Monophosphate-Dependent Protein Kinase¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­57
6. Circular mRNA Encoding for Monomeric and Polymeric Green Fluorescent Protein¡­¡­¡­¡­¡­¡­.69
7. Fluorescence Lifetime Imaging (FLlM) of Green Fluorescent Fusion Proteins in Living Cells¡­¡­89
8. Fluorescence Resonance Energy Transfer (FRET) Applications Using Green Fluorescent Protein¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­101
9. Bioluminescence Resonance Energy Transfer Assays for Protein¨CProtein Interactionsin Living Cells¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­..121
10. Whole-Body Fluorescence Imaging with Green Fluorescence Protein¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­135
11. Drug-Induced Translocation of Nucleolar Proteins Fused to Green Fluorescent Protein¡­¡­..151
12. Light-Induced Nuclear Targetingof PhytochromeB¨CsGreen FluorescentProtein in Plants¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­163
13. Mechanisms of Protein Trafficking¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­..171
14. Analysis of Nucleocytoplasmic Transport Using Green Fluorescent Protein¡­¡­¡­¡­¡­¡­¡­¡­¡­.181
15. Transgenic Bovine Embryo Selection Using Green Fluorescent Protein¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­.201
16. Development of Glycosylphosphatidylinositol-Anchored Enhanced Green Fluorescent Protein¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­215
17. Transgenic Zebrafish Expressing Green Fluorescent Protein¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­.225
18. Transgenic Insects¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­235
19. Green Fluorescent Protein in Transgenic Plants¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­.245
20. Green Fluorescent Protein Calcium Biosensors¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­..255
21. Green Fluorescent Protein Fluobody Immunosensors¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­..265
22. Green Fluorescent Protein-Based Protein Kinase Biosensor Substrates¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­275
23. Green Fluorescent Protein Urea Sensors¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­.287
24. Using Green Fluorescent Protein to Monitor Measles Virus Cell-to-Cell Spreadby Time-Lapse Confocal Microscopy¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­.297
25. Tracking and Selection of Retrovirally Transduced  Murine Bone Marrow Cells Using Green Fluorescent Protein¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­.309
26. Green Fluorescent Protein as a Reporter of Adenovirus-Mediated Gene Transfer and Expression in the Hypothalamic¨CNeurohypophyseal System¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­..321
27. Enhancement of Green Fluorescent Protein Expression in Adeno-Associated Virus with the
Woodchuck Hepatitis Virus Post-Transcriptional Regulatory Element¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­331
28. Construction of Infectious Simian Varicella Virus Expressing Green Fluorescent Protein¡­¡­.341
29. Green Fluorescent Protein in Retroviral VectorConstructs as Marker and Reporter of Gene
Expression for Cell and Gene Therapy Applications¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­353


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  • ¸½¼þ 2 : Contents.doc
  • 2013-07-17 09:22:30, 17.5 K

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