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【答案】应助回帖
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phu_grassman: 金币-10, 多次机器翻译。 2013-05-31 16:43:13
jiangbo9090: 金币+30, 翻译EPI+1, ★★★★★最佳答案, 非常感谢 2013-06-01 07:56:11
全文的翻译的大概是这样的
Fiber raw material is the most abundant on earth now, cheap the important renewable resources, and the application of it has always been the focus of the scientific community and hotspot of research. Cellulase is a highly active biocatalyst. With the development of modern microbiological techniques, cellulase in the feed, alcohol, textile and food etc showed the huge market potential.
Laboratory research on double function of cellulose enzyme CbCel9A1CBM each module function study found its truncated proteins between the progressive and collaborative. Concrete from the relationship of structure - function for further understanding the truncated protein, we want to be able to CbCel 9 and L - a - L CBM3C module proteins in crystal structure analysis, and to fundamentally understand its action mechanism. First of all, we have to target proteins in Ni - column affinity purification, and found in a molecular sieve chromatography purification purification effect is very good. Based on sds-page gel electrophoresis after after a textual research on the purity of target protein with coomassie brilliant blue method of target protein concentrations were determined, and at the same time, concentrated protein concentration. After diffusion method using sessile drop type steam after a large number of experiments, the optimal target protein crystallization conditions were determined and the cultivation of single crystal. Finally concentrated to Shanghai light source of Shanghai institute of applied physics, Chinese academy of science for X-ray synchrotron radiation diffraction resolution crystal structure. |
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