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Clinical efficacy of botanical extracts from Eupatorium adenophorum against the scab mite, Psoroptes cuniculi

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Clinical efficacy of botanical extracts from Eupatorium adenophorum against the scab mite, Psoroptes cuniculi  
×÷Õß: Nong, X (Nong, Xiang)[ 1,2 ] ; Ren, YJ (Ren, Yong-Jun)[ 3 ] ; Wang, JH (Wang, Jia-Hai)[ 1 ] ; Fang, CL (Fang, Chun-Lin)[ 4 ] ; Xie, Y (Xie, Yue)[ 1 ] ; Yang, DY (Yang, De-Ying)[ 1 ] ; Liu, TF (Liu, Tian-Fei)[ 5 ] ; Chen, L (Chen, Lin)[ 1 ] ; Zhou, X (Zhou, Xuan)[ 1 ] ; Gu, XB (Gu, Xiao-Bin)[ 1 ] ; Zheng, WP (Zheng, Wan-Peng)[ 1 ] ; Peng, XR (Peng, Xue-Rong)[ 6 ] ; Wang, SX (Wang, Shu-Xian)[ 1 ] ; Lai, SJ (Lai, Song-Jia)[ 5 ] ; Yang, GY (Yang, Guang-You)[ 1 ]  
À´Ô´³ö°æÎï: VETERINARY PARASITOLOGY  ¾í: 192   ÆÚ: 1-3   Ò³: 247-252   DOI: 10.1016/j.vetpar.2012.10.005   ³ö°æÄê: FEB 18 2013  
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ÕªÒª: This study evaluated the in vivo clinical efficacy of Crofton weed (Eupatorium adenophorum) extracts against the scab mite, Psoroptes cuniculi. A 30-day experiment was performed using New Zealand rabbits that were naturally infested with P. cuniculi on a farm. Rabbits were randomly divided into five groups (6 animals per group); animals in groups A, B and C were treated in each ear topically with 2 ml of 1.0, 0.5 and 0.25 g/ml (w/v) E. adenopho rum ethanol extract, respectively. Animals in groups D and E were treated with ivermectin (by injection; positive controls) and glycerol with water only (by embrocation; negative controls), respectively. Each rabbit was treated twice with separate treatments on days 0 and 7. Rabbits were observed daily and detailed examinations were performed on days 0, 7, 14 and 30, to inspect the presence or absence of mites and scabs/crusts. Clinical infection and the degree of recovery were evaluated, and the rate of reduction in mites and clinical efficacy rate (%) were calculated. The clinical effect of treatment with E. adenopho rum extracts was similar to treatment with ivermectin. Seven days after the initial treatment, the mean clinical scores (presence of scabs/crusts) decreased from 3.32, 3.08 and 3.17 to 0.37, 0.47 and 0.48 in the left ears of animals in groups A, B and C, respectively, and from 3.53, 3.73 and 3.67 to 0.40, 0.45 and 0.48 in the right ears of animals in groups A, B and C, respectively, which were similar to the observations recorded in the positive control rabbits. However, the clinical score for negative control rabbits did not decrease significantly (P > 0.05) during the experiment, and this changed from 3.32 to 2.75 in the left ears and from 3.50 to 3.25 in the right ears, and there were no significant differences in clinical efficacy between left and right ears. After two treatments (7 days space), the rabbits in groups A, B, C and D had recovered completely 30 days after the last treatment and no recurrences of infection were observed. These results indicate that E. adenophorum contains potent compounds for the effective control of animal acariasis. (C) 2012 Elsevier B.V. All rights reserved.  
Èë²ØºÅ: WOS:000314078900030  
ÎÄÏ×ÀàÐÍ: Article  
ÓïÖÖ: English  
×÷Õ߹ؼü´Ê: Acaricidal; P. cuniculi; E. adenophorum; Clinical efficacy  
KeyWords Plus: IN-VITRO; SARCOPTES-SCABIEI; ACARICIDAL ACTIVITY; ESSENTIAL OIL; RABBITS; INFESTATION; POPULATIONS; SELAMECTIN; RESISTANCE; DISEASE  
ͨѶ×÷ÕßµØÖ·: Yang, GY (ͨѶ×÷Õß) Sichuan Agr Univ, Coll Vet Med, Dept Parasitol, Yaan 625014, Sichuan, Peoples R China.
  ÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ
    Sichuan Agricultural University  

µØÖ·:  [ 1 ] Sichuan Agr Univ, Coll Vet Med, Dept Parasitol, Yaan 625014, Sichuan, Peoples R China
  ÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ
    Sichuan Agricultural University  
       [ 2 ] Leshan Normal Univ, Coll Chem & Life Sci, Leshan 614004, Peoples R China
       [ 3 ] Sichuan Acad Anim Sci, Chengdu 610066, Peoples R China
       [ 4 ] Sichuan Engn Res Ctr New Vet Drug, Chengdu 611130, Peoples R China
[ 5 ] Sichuan Agr Univ, Coll Anim Sci & Technol, Yaan 625014, Sichuan, Peoples R China
  ÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ
    Sichuan Agricultural University  
[ 6 ] Sichuan Agr Univ, Coll Life & Basic Sci, Yaan 625014, Sichuan, Peoples R China
  ÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ
    Sichuan Agricultural University  

µç×ÓÓʼþµØÖ·: guangyou1963@yahoo.com.cn  
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Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT)  IRT0848  
Scientific Research Fund of Sichuan Provincial Education Department  11ZB131  

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This study was supported by grants from the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) (No. IRT0848), and the Scientific Research Fund of Sichuan Provincial Education Department (No. 11ZB131). We wish to thank Hai-Han Yang, Chao Li, Zong-Xiu Liu, Yuan-Kun Chen and You-Ping Li (Sichuan Engineering Research Center For New Veterinary Drug, Chengdu, China) for their technical assistance.

³ö°æÉÌ: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS  
Web of Science Àà±ð: Parasitology; Veterinary Sciences  
Ñо¿·½Ïò: Parasitology; Veterinary Sciences  
IDS ºÅ: 078DP  
ISSN: 0304-4017
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×÷Õß: Nong, Xiang; Ren, Yong-Jun; Wang, Jia-Hai; µÈ.
À´Ô´³ö°æÎï: VETERINARY PARASITOLOGY  ¾í: 192   ÆÚ: 1-3   Ò³: 247-252   DOI: 10.1016/j.vetpar.2012.10.005   ³ö°æÄê: FEB 18 2013
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