10楼: Originally posted by jack6335 at 2013-05-25 20:50:26
为了回复帖子凌晨还不休息，多谢多谢啊...

9楼: Originally posted by jack6335 at 2013-05-25 20:48:09
我做的抗体对照在WB之后就只有50kD处有带，你太牛了！...

11楼: Originally posted by 8601 at 2013-05-26 13:40:13
我在国外，你们凌晨是我们中午，呵呵。...

12楼: Originally posted by 8601 at 2013-05-26 13:40:54
那要看你跑的胶了。要是你把25KD的带子跑走了，那哪还看得见。...

14楼: Originally posted by jack6335 at 2013-05-26 13:48:38
胶考染后能看到两条带的！...

15楼: Originally posted by 生物小通 at 2013-05-27 11:38:27
因为抗体的Fc片段由重链组成，再加上那个部位与种属相关（好似是，记不清了），所以一般的二抗就是抗这个部位的。...

8楼: Originally posted by 8601 at 2013-05-24 01:02:37
我们实验室有一种二抗叫 conjugated proteinA. 这种抗体据老板说只识别有活性的抗体不识别变性的。但我做实验的经验是如果免疫沉淀时放的抗体比较多，还是会识别的，但确实要比一般的弱些。...