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金狮水友至尊木虫 (著名写手)
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[求助]
求英译汉---生物传感---177字80金币-----一---翻译好可以再加金币
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| MicroRNAs (total RNA) in cultured cancer cells were extracted using a RNA extraction kit from Qigen according to the recommended procedure, and miRNAs in the total RNA were enriched by using a cleanup kit (Qigen). Circulating miRNAs in serum were extracted using the miRNeasy RNA isolation kit from Qiagen. Briefly, 5 mL of Qiazol solution was added to 0.50 mL of serum. The mixture was thoroughly mixed and incubated for 10 min at room temperature. After 1.0 mL of chloroform was added, the mixture was vortexed for 60 s and centrifuged at 14 000 g for 15 min at 4 °C. Isolation and purification of miRNAs in the aqueous phase was achieved following the recommended procedure. Finally, miRNAs in the extracted total RNA samples were analyzed as described above. Reference values of miRNAs were obtained by qPCR (the gold standard). Direct profiling of circulating miRNAs in serum was conducted after spiking aliquots of concentrated TMD buffer to serum samples to have a final TMD buffer composition of 50 mM Tris-HCl, 5.0 mM MgCl2, and 1.0 mM DTT. |
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2楼2013-05-15 16:19:08
金狮水友
至尊木虫 (著名写手)
- 应助: 9 (幼儿园)
- 金币: 12278.2
- 散金: 50
- 红花: 13
- 沙发: 5
- 帖子: 1686
- 在线: 203小时
- 虫号: 1817809
- 注册: 2012-05-15
- 性别: GG
- 专业: 功能与智能高分子
呵呵，可以加金币的哦 3楼2013-05-16 20:29:02
金狮水友
至尊木虫 (著名写手)
- 应助: 9 (幼儿园)
- 金币: 12278.2
- 散金: 50
- 红花: 13
- 沙发: 5
- 帖子: 1686
- 在线: 203小时
- 虫号: 1817809
- 注册: 2012-05-15
- 性别: GG
- 专业: 功能与智能高分子
4楼2013-05-16 20:29:26
yili_90
金虫 (正式写手)
- 翻译EPI: 17
- 应助: 37 (小学生)
- 贵宾: 0.013
- 金币: 3136.5
- 散金: 400
- 红花: 11
- 帖子: 464
- 在线: 117.7小时
- 虫号: 2157293
- 注册: 2012-11-30
- 性别: MM
- 专业: 动物遗传学
【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
金狮水友: 金币+122, 翻译EPI+1, ★★★★★最佳答案, 谢谢美女帮忙，3Q 2013-05-16 21:41:34
爱与雨下: 金币+1 2013-05-16 21:46:59
金狮水友: 金币+122, 翻译EPI+1, ★★★★★最佳答案, 谢谢美女帮忙，3Q 2013-05-16 21:41:34
爱与雨下: 金币+1 2013-05-16 21:46:59
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5楼2013-05-16 21:34:52