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As compared to other miRNA assays, the proposed electrochemical biosensor exhibited several distinct advantages: (1) the simplicity of the one-step label-free detection; (2) isothermal amplification process; (3) excellent sensitivity, selectivity; and (4) the freedom from miRNA labeling and thermal amplification. It greatly improves the suitability in direct profiling miRNA expressions with minimal or no sample pretreatments. The proposed biosensor is therefore an attractive candidate for the development of a simple, robust, and ultrasensitive miRNA expression profiling platform for uses at point-of-care. |
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