|
|
【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★
liujie4567: 金币+10, 基金HEPI+1, ★★★★★最佳答案, HEPI 2014-06-09 09:33:49
标题：针灸对截瘫兔损伤脊髓神经再生营养修复影响的机理研究
中文摘要：目前未见兔脊髓神经元的体外培养成功的报导.我们参考鼠神经元原代培养的方法，培养兔脊髓神经元,作为针灸治疗损伤脊髓神经元营养修复的主要研究方法。
在既往的研究中，我们指出了针灸对截瘫损伤段的作用可能是：a.促使加速神经纤维再生；b.防止瘢痕形成以使神经纤维顺利通过；c.促使休眠的神经元早日苏醒、受损伤的神经元的核蛋白合成等有关。
本篇研究通过针灸截瘫兔后对脊髓神经元用NGF，bfgf，MMP进行培养，从细胞凋亡和凋亡途径相关因子FADD、caspase-8、cytochrom C、caspase-9、 caspas-3的表达差异，细胞信号转导途径的差异（包括H3Tdr测量、Ca2+浓度、细胞活力即MTT分析光量度OD值、胞体轴突胞体直径）,铁超载与转运包括(铁含量、AQP4表达)、脊髓氧化损伤(MDA／Cu．Zn．SOD)与铁转运蛋白等变化差异, 探讨针灸对损伤脊髓神经元再生营养修复的机理。
中文关键词：1.针灸治疗截瘫兔；2.脊髓神经再生营养修复；3.细胞凋亡；4.细胞信号传递；5.铁超载与转运.
The research is mechine about acupuncture and moxibustion to paralysed rabbits on nerve regeneration influcnce of nutritional rehabilitation after spinal cord injury.
It is now ,No rabbit spinal cord neurons in vitro success reports , we refer to Big mouse neurons in primary culture method, cultured rabbit spinal cord neurons, as acupuncture treatment spinal cord injury neurons nutritional rehabilitation main research methods
In previous studies, we pointed out acupuncture to paraplegia damaged section role may be: a. to promote accelerated nerve fiber regeneration; b. to prevent scar formation in the nerve fibers pass; c. promote an early wake up dormant neurons, promote by damage neuronal in nuclear protein synthesis
This study is acupuncture to paraplegia rabbits spinal cord neurons with NGF, bfgf, MMP were cultured, on apoptosis and apoptosis-related factors FADD, caspase-8, cytochrom C, caspase-9, caspas-3 expression differences , cell signal transduction pathways differ (including H3Tdr measurement, Ca2 + concentration, cell viability that MTT assay optical measuring OD value, the cell body axon cell body diameter), iron overload and transportation, including (iron content, AQP4 expression), spinal cord oxidative damage (MDA / Cu.Zn.SOD) and Iron transport protein changes differences , Discussion the mechanism of acupuncture to spinal cord injury neuronal regeneration and nutritional rehabilitation.
Keywords:1.Acupuncture treatment paraplegia Rabbit;2.spinal cord injury neuronal regeneration and nutritional rehabilitation;3.Apoptosis;4.Cell signaling transduction;5.Iron overload and transportation. |
|
回复此楼