| 查看: 315 | 回复: 0 | ||
maliyuan0924铁虫 (小有名气)
|
[求助]
摘要 英译汉
|
|
题目The type and extent of injuries in vitrified mouse oocytes 摘要To improve the vitrification of mouse oocytes using straws, we attempted to estimate the type and extent of injuries during vitrification with a vitrification solution EAFS10/10. Injuries in oocytes were assessed based on cellular viability, the integrity of the plasma membrane, the status of the meiotic spindle/chromosomes, and morphological appearance. For morphologically normal oocytes, the ability to be fertilized and to develop into blastocysts was examined. Morphological assessment revealed 15% of oocytes to be injured by intracellular ice formed during vitrification, and 10% by osmotic swelling during removal of the cryoprotectant. When assessed by the status of spindles/chromosomes, the most sensitive criterion, damage was found in 16% of oocytes without any treatment. This value was similar to the proportion of fresh oocytes that did not cleave after insemination (13%). On exposure to EAFS10/10, the spindles/chromosomes were affected in 33% of oocytes. The exposure reduced the rate of cleavage by 18% points and the rate of development into blastocysts by 19 points. Vitrification reduced these rates by 15% and 36% points, respectively. Although the mechanism responsible for this moderate toxic effect on developmental ability is not known, information obtained in the present study will be useful to develop a practical method for the vitrification of mouse oocytes using straws.  YBD{@7}JN[~E(S[9JJLGSK3.jpg |
回复此楼» 猜你喜欢
今年审到国自然15份，谈谈感受
已经有20人回复
-
Sci. Bull. 悲剧经验
已经有5人回复
-
国自然评分
已经有4人回复
-
提交了我也来说说感想
已经有4人回复
-
面上本子正文33页，违规吗？会被低分嘛？
已经有11人回复
-
评审有感
已经有22人回复
-
反应很差，大量原料没有反应
已经有6人回复
-
如果工作了想读博，可以边工作边读全日制嘛？
已经有5人回复
-
如何实现卤原子转化
已经有8人回复
-
河北省自然科学基金
已经有3人回复
5