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wt07060210新虫 (小有名气)
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[求助]
重金属铜对芹菜幼苗生长的影响
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本实验的目的在于研究重金属铜对芹菜的生理毒性及硅的缓解作用。 采用不同浓度硫酸铜（20,40,60,80,100 mg/L）处理芹菜幼苗，测定了幼苗植株生长状况、光合作用指标和抗氧化活性；并采用20,40,60,80,100 mg/L 硅酸钠处理经硫酸铜危害的幼苗，测定硅对重金属铜的缓解作用。 随着铜处理浓度的升高，芹菜幼苗的生长抑制增强，生物学产量下降。叶绿素a和叶绿素b含量与对照相比显著降低。60 mg/L 硫酸铜处理幼苗的过氧化物酶和超氧化物歧化酶活性最高。40 mg/L 的硅酸钠对硫酸铜的解毒作用最为明显。 高浓度的硫酸铜抑制芹菜的幼苗生长，降低光合作用能力。硫酸铜处理后植株幼苗可以产生一定的生理应答，适当浓度的硅处理可以缓解重金属铜的危害。 [ Last edited by wt07060210 on 2013-5-8 at 15:04 ] |
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wt07060210
新虫 (小有名气)
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5楼2013-05-08 16:25:46
nwsuafliu
木虫 (著名写手)
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【答案】应助回帖
| The study aimed to investigate the physiological toxicity effect of heavy metal copper on celery seedlings and its alleviation by silicon treatment. Celery seedlings were treated with CuSO4 at various concentrations (20, 40, 60, 80, 100 mg/L) and measured for their growth, photosynthetic parameters and antioxidant activities. Na2SiO3 solutions in the same concentration range (20, 40, 60, 80, 100 mg/L) were applied on the CuSO4-treated seedlings to verify the alleviation effects of silicon. As a result, the growth retardation of celery seedlings was enhanced and biological yield was reduced with the increase of copper concentration. The content of chlorophyll a and b were significantly lower than that of control. The highest enzymatic activity of SOD and POD was found in seedlings treated with 60 mg/L CuSO4. Meanwhile, the best detoxification effect was exerted by 40 mg/L Na2SiO3. In conclusion, high concentration of CuSO4 inhibited the growth of celery seedlings and compromised their photosynthetic machinery. Celery seedlings showed some physiological response to CuSO4 treatment and silicon treatment at proper concentration might alleviate the harmful effect of copper. |
2楼2013-05-08 15:57:17
wt07060210
新虫 (小有名气)
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3楼2013-05-08 16:19:02
nwsuafliu
木虫 (著名写手)
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- 专业: 作物分子育种
4楼2013-05-08 16:23:24
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