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Microbial Enzymes and Biotransformations
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Publisher: Humana Press Number Of Pages: 336 Publication Date: 2005-01-19 ISBN / ASIN: 1588292533 Book Description: Leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for freeduan.com human health, nutrition, and environmental protection. Overview chapters on microorganisms as a source of metabolic and enzymatic diversity, and on the fast-moving field of enzyme biosensors are presented. Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications. Contents Preface ..............................................................................................................v Contributors ..................................................................................................... ix 1 Microbial Cells and Enzymes: A Century of Progress José L. Adrio and Arnold L. Demain ..................................................... 1 2 Enzyme Biosensors Steven J. Setford and Jeffrey D. Newman .......................................... 29 3 Directed Evolution of Enzymes: How to Improve Nucleic Acid Polymerases for Biocatalysis Susanne Brakmann and Sascha Nico Stumpp ..................................... 61 4 L-Glutaminase as a Therapeutic Enzyme of Microbial Origin Abdulhameed Sabu, K. Madhavan Nampoothiri, and Ashok Pandey ..... 75 5 Enzymatic Production of D-Amino Acids Hemraj S. Nandanwar, Gurinder S. Hoondal, and Rakesh M. Vohra .... 91 6 Screening of Microorganisms for Enzymatic Biosynthesis of Nondigestible Oligosaccharides Oskar Ba?uelos, Maria del Carmen Ronchel, José Luis Adrio, and Javier Velasco ................ 105 7 Food-Grade Corynebacteria for Enzyme Production José A. Gil, Angelina Ramos, Sirin A. I. Adham, Noelia Valbuena, Michal Letek, and Luis M. Mateos ................... 115 8 Enzymatic Synthesis of (S)-Phenylalanine and Related (S)-Amino Acids by Phenylalanine Dehydrogenase Yasuhisa Asano ................................................................................. 141 9 Enzymes in Modern Detergents Susumu Ito, Tohru Kobayashi, Yuji Hatada, and Koki Horikoshi ..... 151 10 Microbial Proteases Chandran Sandhya, K. Madhavan Nampoothiri, and Ashok Pandey ... 165 11 Proteases Produced by Halophilic Bacteria and Archaea Encarnación Mellado, Cristina Sánchez-Porro, and Antonio Ventosa .... 181 12 Microbial Pectinases Rupinder Tewari, Ram P. Tewari, and Gurinder S. Hoondal ........... 191 13 Expression of Microbial Phytases in Yeast Systems and Characterization of the Recombinant Enzymes Xin Gen Lei and Taewan Kim ........................................................... 209 14 Glucose Dehydrogenase for the Regeneration of NADPH and NADH Andrea Weckbecker and Werner Hummel ...................................... 225 15 Acetate Kinase from Methanosarcina thermophila, a Key Enzyme for Methanogenesis Prabha Iyer and James G. Ferry ........................................................ 239 16 Immobilization of Enzymes by Covalent Attachment Scott J. Novick and J. David Rozzell ................................................ 247 17 Preparation of an Industrial Biocatalyst of Penicillin G Acylase on Sepabeads: Improving the Design of Penicillin G Hydrolysis Cesar Mateo, Valeria Grazu, Olga Abian, Manuel Fuentes, Gloria Fernández-Lorente, José M. Palomo, Roberto Fernández-Lafuente, and José M. Guisán....................... 273 18 Methods for Bioencapsulation of Enzymes and Cells Thomas M. S. Chang ......................................................................... 289 Index ............................................................................................................ http://media.imhb.cn/myspace/download.aspx?MSAutoID=64636&huid=460586 |
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