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[资源] Applied Thin-Layer Chromatography: Best Practice and Avoidance of Mistakes

By Elke Hahn-Deinstrop (Author)
Publisher:  Wiley-VCH
Number Of Pages:  330
Publication Date:  2007-01-09
ISBN / ASIN:  3527315535

Book Description:
Thin layer chromatography (TLC) is a powerful, fast and inexpensive analytical method. It has proven its usefulness in pharmaceutical, food and environmental analysis. This new edition features a completely revised chapter on documentation, now including the use of digital cameras. Selected new sorbents and instruments are also introduced.
Why has the prior edition been successful? All steps of the analytical procedure are clearly explained, starting with the choice of a suitable TLC technique and ending with data evaluation and documentation. Special emphasis is put on the proper choice of materials for TLC. Properties and functions of various materials and the TLC equipment are described, covering e. g. precoated layers, solvents and developing chambers, and including information on suppliers. Many practical hints for trouble shooting are given. All this is illustrated with numerous coloured figures.
How to use TLC in compliance with GLP/GMP regulations is described in detail, including the required documentation. Therefore the reader can very easily compile his own standard operating procedures.

Introduction.
1.1 What Does TLCMean?

1.2 When Is TLC Used?

1.3 Where Is TLC Used?

1.4 How Is the Result of a TLC Represented?

1.4.1 Retardation Factor.

1.4.2 Flow Constant.

1.4.3 Other TLC Parameters.

1.5 What Kinds of Reference Substances Are Used in TLC?

1.6 The Literature on TLC.

1.6.1 General Literature.

1.6.1.1 Books and Information Sheets in German.

1.6.1.2 Books in English.

1.6.1.3 Book in Another Language.

1.6.2 Journals.

1.6.2.1 German Language Journals Containing Articles on TLC (Selection).

1.6.2.2 English Language Journals on TLC.

1.6.2.3 English Language Journals Containing General Articles on Chromatography (Selection).

1.6.3 Abstracts.

1.6.4 Pharmacopoeias.

2 Precoated Layers.

2.1 Precoated Layers –Why?

2.2 What Are Precoated Layers Produced?

2.2.1 Sorbents.

2.2.2 Supports for Stationary Phases.

2.2.3 Additives.

2.3 What Types of Precoated Layers Are There?

2.4 What Are the Uses of Precoated Layers?

2.5 Criteria for the Selection of Stationary Phases in TLC.

2.5.1 How Can the Choice of the Stationary Phase beMade?

2.5.2 How Can the Recommendations for Stationary Phases Found in Pharmacopoeias be Applied to Precoated Layers?

2.6 Effect of the Stationary Phase When Mobile Phases Are Identical.

2.7 Advice on the Ordering and Storage of Precoated Layers.

2.8 Problems in the Naming and Arrangement of Precoated Layers.

3 Before the TLC Development Process.

3.1 Handling of Precoated Layers.

3.1.1 Film and Foil.

3.1.2 Glass Plates.

3.2 Prewashing.

3.3 Activation.

3.4 Conditioning.

3.5 Impregnation.

3.5.1 Impregnation by Dipping.

3.5.2 Impregnation by Spraying.

3.5.3 Impregnation by Predevelopment.

3.6 Application of Samples.

3.6.1 Manual Application of Samples.

3.6.2 Semiautomatic Application.

3.6.3 Fully Automatic Application.

3.7 Positioning of the Samples.

3.8 Drying Before the Development.

4 Solvent Systems, Developing Chambers and Development.

4.1 Solvent Systems.

4.1.1 Choice of Solvent Systems.

4.1.2 Preparation and Storage of Solvent Systems.

4.1.3 Problematical Solvent System Compositions.

4.2 TLC Developing Chambers.

4.2.1 What Types of TLC Developing Chambers Are There?

4.2.1.1 TLC Chambers for Vertical Development.

4.2.1.2 TLC Developing Chambers for Horizontal Development.

4.2.2 Influence of the Chamber Atmosphere.

4.2.2.1 The Unsaturated N-Chamber.

4.2.2.2 The Saturated N-Chamber.

4.2.3 Influence of Temperature in Chromatography.

4.2.4 Location and Labeling of TLC Developing Chambers.

4.3 Development of Thin-Layer Chromatograms.

4.3.1 One-Dimensional Thin-Layer Chromatography.

4.3.1.1 Vertical Development.

4.3.1.2 Horizontal Development.

4.3.2 Two-Dimensional Thin-Layer Chromatography.

4.4 Drying After Development.

5 Evaluation Without Derivatization.

5.1 Direct Visual Evaluation.

5.1.1 Detection in Daylight.

5.1.2 Detection with 254-nm UV Light.

5.1.3 Detection with 365-nm UV Light.

5.2 Direct Optical Evaluation Using Instruments.

5.2.1 Principle of Operation of a TLC Scanner.

5.2.2 Direct Optical Evaluation Above 400 nm.

5.2.3 Direct Optical Evaluation Below 400 nm.

5.2.4 Direct Optical Evaluation with 365-nm UV Light (FluorescenceMeasurement).

5.3 Diode-Array Detection.

5.4 Coupled Methods for Substance Identification.

5.5 Documentation Without or Before Derivatization.

6 Derivatization.

6.1 Thermochemical Reaction.

6.2 Irradiation with High-Energy Light.

6.3 Reaction with Reagents.

6.3.1 Spraying of TLC Plates.

6.3.1.1 Manual Spraying of TLC Plates.

6.3.1.2 Fully Automatic Spraying of TLC Plates.

6.3.2 Dipping of TLC Plates.

6.3.3 Vapor Treatment of TLC Plates.

6.3.4 Coating TLC Plates.

6.4 Special Cases of Derivatization.

6.4.1 Prechromatographic Derivatization.

6.4.1.1 Reaction with Reagents.

6.4.1.2 Incorporation of Radionuclides.

6.4.2 Simultaneous Derivatization and Development.

6.4.3 Reaction Sequences.

6.4.4 Biological-Physiological Methods of Detection.

6.5 Further Treatment of Derivatized Chromatograms.

6.5.1 Effect of Heat.

6.5.2 Stabilization of Colored and Fluorescent Zones.

7 Evaluation After Derivatization.

7.1 Visual Evaluation.

7.1.1 Visual Qualitative Evaluation.

7.1.2 Visual Semiquantitative Evaluation.

7.2 Evaluation Using a TLC Scanner.

7.2.1 Qualitative Evaluation.

7.2.2 Quantitative Evaluation.

7.2.2.1 Absorption Measurement.

7.2.2.2 Fluorescence Measurement.

7.2.2.3 Comparison of  “Parallel” With “Transverse” Measurement.

7.3 Evaluation Using a Video System.

7.3.1 Qualitative Video Evaluation.

7.3.2 Quantitative Video Evaluation.

7.3.3 Comparison of the TLC Scanner With Video Evaluation.

7.4 Evaluation by Flat-Bed Scanner.

7.5 Evaluation Using a Digital Camera.

8 Documentation.

8.1 Description of a Thin-Layer Chromatogram.

8.2 Documentation by Drawing, Tracing and Photocopying.

8.3 Photographic Documentation.

8.3.1 Photography Using the Polaroid Camera MP-4.

8.3.2 Photography Using 35-mm Cameras.

8.3.2.1 Photography in 254-nm UV Light.

8.3.2.2 Photographs in 365-nm UV Light.

8.3.2.3 Photographs inWhite Light.

8.3.3 Archiving of 35-mm Films.

8.4 Video Documentation.

8.5 DocumentationWith Digital Cameras.

8.6 TLC Scanner Documentation.

8.7 Flat-Bed Scanner Documentation.

8.8 Bioluminescence Measurements.

8.8.1 Toxicity Screening Using the Bioluminescent Bacteria Vibrio fischeri.

8.8.2 Detecting Bioluminescence With the BioLuminizer.

9 GMP/GLP-Conforming Operations in TLC.

9.1 Validation of TLC Methods.

9.2 Use of Qualified/Calibrated Equipment.

9.3 GMP/GLP-Conforming Raw Data Sheets.

9.4 Examples of GMP/GLP-Conforming Testing Procedures (TPs).

9.4.1 Identity and Purity of a Bulk Pharmaceutical Chemical and Determination of the Limit Values of Related Compounds.

9.4.2 Identity and Purity of Various Flavonoid-Containing Plant Extracts.

9.4.3 Content of a Pharmaceutical Chemical in a Tablet.

10 Effects of Stress.

10.1 Controlled Stress on a Substance.

10.2 TLC-Sensitive Substances.

10.2.1 Interactions With Sorbents.

10.2.2 Effect of Elevated Temperature.

10.2.3 Effect of Light.

10.2.4 Oxidative Effects.

11 Special Methods in TLC.

11.1 AMD – AutomatedMultiple Development.

11.2 OPLC – Overpressured Layer Chromatography.

11.3 HPPLC – High Pressure Planar Liquid Chromatography.

11.4 TLC-FID/FTID – Combination of TLC and Flame-Ionization Detector or Flame-Thermionic Ionization Detector.

11.5 TLC-NDIR.

11.6 RPC – Rotation Planar Chromatography.

12 Appendix.

12.1 CHROMart.

12.2 References.

12.3 Abbreviations.

12.4 Acknowledgements.

12.5 Market Overview.

Photograph Section.

Subject Index.
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