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aft.1988

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[求助] 如何判断动态猝灭还是静态猝灭

做药物与HSA的荧光实验，Ksv随着温度的升高增大，但是Kq却达到10的12次方远大于10的10次幂，怎么判断究竟是动态猝灭还是静态猝灭？有相应的参考文献吗？紫外能够辅助判断静态猝灭还是动态猝灭吗？求助各位高手！

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1楼 2013-04-30 11:20:49

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283279631

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【答案】应助回帖

刚看到一篇类似的文献，人家是这样解释的：囧~~
The plots show a good linear relationship within the investigated concentrations. The results showed that Kq were much greater than the limiting diffusion rate constant of the biomolecule (2.0 × 10-10L mol−1S−1), which indicated that the probable quenching mechanism of BSA–IMTZ interactions was initiated by complex formation rather than by dynamic collision. While the results also showed that the values of Stern–Volmer quenching constants KSV and Kq increased with increasing tem-perature, it indicated that the probable quenching mechanism of BSA–IMTZ interactions was initiated by dynamic collision [19,20] .In other words, the fluorescence quenching of BSA results from complex formation, while that dynamic collision could be not neg-ligible.

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aft.1988

2楼2013-06-13 11:03:31

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aft.1988

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2楼: Originally posted by 283279631 at 2013-06-13 11:03:31
刚看到一篇类似的文献，人家是这样解释的：囧~~
The plots show a good linear relationship within the investigated concentrations. The results showed that Kq were much greater than ...

能否提供这篇文献？谢谢！

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3楼2013-06-14 09:15:42

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