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xy656691金虫 (正式写手)
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Among all the arf mutants, arf2, arf3, arf6, and arf8 were not auxin resistant whereas arf5, arf7, and arf19 are known to have decreased sensitivities to exogenous auxin. Loss-of-function mutations in several ARFs have been isolated from forward screens for developmental mutants or reverse genetic screens for T-DNA insertion mutants Herein we present the isolation of novel alleles of arf19 and arf7 mutants by screening for mutants resistant to sirtinol, a synthetic drug that was shown to activate auxin signaling pathways without interfering with the auxin polar transport。 Finally, we found that an ARF19 genomic fragment not only complemented arf19 mutations, it also rescued arf7 in terms of auxin resistance, nonphototropic responses, and adult plant phenotypes. These results suggest that ARF19 can be substituted for ARF7 at the protein level and the different phenotypes observed for arf7 and arf19 are probably caused by the differential expression patterns and levels of the two genes. [ Last edited by xy656691 on 2013-4-19 at 11:48 ] |
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huangzx1314
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xy656691: 金币+2, 翻译EPI+1 2013-04-19 12:40:44
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xy656691: 金币+2, 翻译EPI+1 2013-04-19 12:40:44
爱与雨下: 翻译EPI-1 2013-04-27 20:30:10
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Among all the arf mutants, arf2, arf3, arf6, and arf8 were not auxin resistant whereas arf5, arf7, and arf19 are known to have decreased sensitivities to exogenous auxin 在所有的arf突变体中，arf2, arf3, arf6, 和arf8不具有植物生长素抵抗性，然而arf5, arf7,和arf19被认为具有降低对外源生长素的敏感性。 |
2楼2013-04-19 12:02:39
xy656691
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3楼2013-04-19 12:40:38
huangzx1314
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4楼2013-04-19 12:42:08
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xy656691: 金币+3 2013-04-27 19:09:06
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5楼2013-04-26 19:22:28
xy656691
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6楼2013-04-27 19:08:59
