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A novel lumbricin-like antimicrobial peptide named lumbricin-PG was isolated from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen), using a procedure of one step Sephadex G-50 gel filtration and one step C8reversephase high-performance liquid chromatography (RP-HPLC). Its aminoacid sequence was determined as FSRYARMRDSRPWSDRKNNYSGPQFTYPPEKAPPEKLIKWNN EGSPIFEM-PAEGGHIEP by Edman degradation combined with cDNA cloning and mass spectrometry analysis. The cDNA encoding lumbricin-PG was cloned by cDNA library screening. The predicted protein from the cDNA sequence was composed of 73 amino acid residues including a mature lumbricin-PG and predicted signal peptide. It showed similarity with lumbricin antimicrobial peptide from the earthworm, Lumbricus rubellus by BLAST search. Purified lumbricin-PG exerted potential antimicrobial activities against bacteria and fungi; it showed weak hemolysis activity against human and rabbit red cells. |
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huangzx1314
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嗰叽嗰叽: 金币+10, 翻译EPI+1, ★有帮助, 多处不准确，但还是谢谢你！ 2013-04-18 15:42:49
sltmac: 金币+10 2013-06-12 09:52:30
嗰叽嗰叽: 金币+10, 翻译EPI+1, ★有帮助, 多处不准确，但还是谢谢你！ 2013-04-18 15:42:49
sltmac: 金币+10 2013-06-12 09:52:30
| 一种被称作蚯蚓素-PG的新蚯蚓状抗菌肽从一种称作廉环毛蚓的蚯蚓表皮分泌物中分离出来，分离过程是通过一步交联葡聚糖G-50溶胶过滤以及一步C8逆相高效液相层析法（简称：RP-HPLC）实现的。它的氨基酸序列被Adman通过结合cDNA克隆（cDNA编码通过cDNA库筛选的方法进行）和质谱分析确定为FSRYARMRDSRPWSDRKNNYSGPQFTYPPEKAPPEKLIKWNN EGSPIFEM-PAEGGHIEP，。目前认为cDNA蛋白质序列由73个氨基酸残基组成，包括一种自然的蚯蚓素-PG以及信号肽。结果显示这种信号肽与BLAST研究组发现的粉正蚓体内的抗菌肽相似。提纯后的蚯蚓素-PG具有潜在的抗活性（包括真菌和细菌）；它对人体以及兔子红细胞具有弱的溶血活性。 |
2楼2013-04-18 08:59:36
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sltmac: 金币+10 2013-06-12 09:52:35
sltmac: 金币+10 2013-06-12 09:52:35
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3楼2013-04-26 19:30:56
