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小雨莎莎

木虫 (正式写手)

[求助] 咨询关于SPR检测解离常数

各位虫友大家好,我想咨询一下,有虫友用SPR方法检测解离常数的么?要怎么测呢?我们用的是GE healthcare 的Biacore3000.这个仪器有现成的检测解离常数的程序么?
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小雨莎莎

木虫 (正式写手)

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4楼: Originally posted by robustli at 2013-04-15 05:08:06
According to my memory, I used 300s to determine the dissociation constant.

Keep elution using PBS for 300s? Or do you have any detailed protocol? Thanks!
5楼2013-04-15 11:07:58
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robustli

银虫 (小有名气)

【答案】应助回帖

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感谢参与,应助指数 +1
wizardfan: 金币+2, 谢谢参与 2013-04-13 08:16:54
小雨莎莎: 金币+1, ★有帮助 2013-04-13 11:00:16
I have done this. Use the off-line software to calculate the dissociation factor. Refer the manual.
immunologyparasite
2楼2013-04-13 06:16:25
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小雨莎莎

木虫 (正式写手)

引用回帖:
2楼: Originally posted by robustli at 2013-04-13 06:16:25
I have done this. Use the off-line software to calculate the dissociation factor. Refer the manual.

Can I determine the dissociation constant by elution for a period of time?
3楼2013-04-13 11:00:10
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robustli

银虫 (小有名气)

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小雨莎莎: 金币+1, ★★★很有帮助 2013-04-15 11:06:01
According to my memory, I used 300s to determine the dissociation constant.
immunologyparasite
4楼2013-04-15 05:08:06
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