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yin198716гæ (ÕýʽдÊÖ)
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Tumor-bearing mice were injected i.v. with 2 mg pimonidazole (Hypoxylprobe-1) in 0.9% saline, 100 A g biotinylated tomato lectin (Vector Laboratories), or 0.6 mg Hoechst 33342 in PBS 30 min,10 min, or 1 min before sacrifice of the mouse, respectively. Paraffinembedded tissue blocks from formalin-fixed tumor samples were sectioned,dewaxed, and rehydrated using standard techniques. For immunofluores-cent staining, 7-¦Ìm cryo-sections were fixed in acetone for 10 min and dried in air for 1h. |
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xzx8066
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- ·ÒëEPI: 29
- Ó¦Öú: 0 (Ó×¶ùÔ°)
- ½ð±Ò: 2623.7
- ºì»¨: 2
- Ìû×Ó: 434
- ÔÚÏß: 89.1Сʱ
- ³æºÅ: 1350357
- ×¢²á: 2011-07-19
- ÐÔ±ð: GG
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phu_grassman: ½ð±Ò+2, thanks for your help 2013-04-01 08:38:19
yin198716: ½ð±Ò+10, ·ÒëEPI+1, ¡ïÓаïÖú 2013-04-01 08:51:26
phu_grassman: ½ð±Ò+2, thanks for your help 2013-04-01 08:38:19
yin198716: ½ð±Ò+10, ·ÒëEPI+1, ¡ïÓаïÖú 2013-04-01 08:51:26
| ·Ö±ðÓÚ´¦ËÀСÊóǰ30¡¢10ºÍ1·ÖÖÓ£¬½«2 mgÍâÔ´ÐÔÉúÎﻹԵÍÑõ±ê¼ÇÎïßßĪÏõßò(ÈÜÓÚ0.9%ÑÎË®)¡¢100 mgÉúÎïËØ±ê¼ÇµÄ·¬ÇÑÄý¼¯ËØ(Vector Laboratories¹«Ë¾)ºÍ0.6 mg DNAȾÁÏHoechst 33342(ÈÜÓÚPBS)¾¾²Âö×¢Éäµ½ÔØÁöСÊóÌåÄÚ¡£´Ó¸£¶ûÂíÁ̶ֹ¨µÄÖ×Áö±ê±¾ÖзÖÀë³öÀ´µÄ×éÖ¯¿é£¬¾Ê¯À¯°üÂñºó£¬°´±ê×¼²Ù×÷²½ÖèÇÐÆ¬¡¢ÍÑÀ¯¡¢Ë®»¯¡£½øÐÐÃâÒß×黯Ⱦɫʱ£¬7 ¦Ìm±ù¶³ÇÐÆ¬Óñûͪ¹Ì¶¨10·ÖÖÓ£¬È»ºó¿ÕÆø¸ÉÔï1Сʱ¡£ |
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