24小时热门版块排行榜    

查看: 759  |  回复: 0

louise0220

新虫 (正式写手)

[求助] Lowry法测定腐植酸问题求解答

我找到了一篇早期的文献,里面有一段描述用Lowry法测定腐植酸和蛋白质的过程。但是我不是很理解啊。什么叫硫酸铜加不加,分别能测定蛋白质和腐殖酸啊?
Proteins and humic compounds were deter-mined using the Lowry procedure.Colour development was mea- sured with and without CuSO4 addition. When CuSO4 is omitted the colour development is due to humic compounds (Box 1983) and chromogenic amino acids (Peterson 1979). Without CuSO4 the colour developed by bovine serum albumin (BSA) decreased to 20% but no decrease was observed for humic acids. The mu- tual  interference  of proteins and humic compounds was ad-dressed as shown in Eqs. 1-4.
哪位神仙有用过此方法测定腐植酸的吗?如果有的话,能不能告知一二,或者如果有该方法比较详细的资料,也可以发一份给我参考一下。。。谢谢啊。。。。

» 猜你喜欢

已阅   关注TA 给TA发消息 送TA红花 TA的回帖

智能机器人

Robot (super robot)

我们都爱小木虫

找到一些相关的精华帖子,希望有用哦~

科研从小木虫开始,人人为我,我为人人
相关版块跳转 我要订阅楼主 louise0220 的主题更新
信息提示
请填处理意见