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专业: 神经生物学

[求助] J12100011/J0109国家基础科学人才培养基金

求助摘要的编号:

J12100011/0109

项目名称:

针灸对截瘫兔损伤脊髓NGF、BFGF、MMP抗瘢痕抗休眠的机理研究

项目类型:

国家基础科学人才培养基金

中文摘要:
1.目前未见兔脊髓神经元的体外培养成功的报导.我们参考鼠神经元原代培养的方法，培养兔脊髓神经元。
2.在既往的研究中，我们指出了针灸对截瘫损伤段的作用可能是：a.促使加速神经纤维再生；b.防止瘢痕形成以使神经纤维顺利通过；c.促使休眠的神经元早日苏醒、受损伤的神经元的核蛋白合成等有关。
  本篇研究通过针灸截瘫兔后对脊髓神经元用NGF，bfgf，MMP进行培养，从细胞信号转导途径的差异（包括H3Tdr测量、Ca2+浓度、细胞活力即MTT分析光量度OD值、胞体轴突胞体直径）,细胞凋亡和凋亡途径相关因子FADD、caspase-8、cytochrom C、caspase-9、 caspas-3的表达差异，探讨针灸对损伤脊髓神经元抗瘢痕、抗休眠的机理.

中文关键词:1.针灸治疗截瘫;2.兔脊髓神经元体外培养;3.NGF, bfgf，MMP;4.抗瘢痕;5.抗休眠.

英文摘要:
1.It is now ,No spinal cord neurons in vitro success  reports , we refer to Big  mouse  neurons in primary culture method, cultured rabbit spinal cord neurons
2.In previous studies, we pointed out acupuncture to paraplegia  damaged section  role  may be: a. to promote accelerated nerve fiber regeneration; b. to prevent scar formation in the nerve fibers pass; c. promote an early wake up dormant neurons, promote  by damage neuronal  in nuclear protein synthesis.
This study is acupuncture  to  paraplegia rabbits spinal cord neurons with NGF, bfgf, MMP were cultured, on  cell signal transduction pathways differ (including H3Tdr measurement, Ca2 + concentration, cell viability that MTT assay optical measuring OD value, the cell body axon cell body diameter),apoptosis and apoptosis-related factors FADD, caspase-8, cytochrom C, caspase-9, caspas-3 expression differences , Discussion the mechanism  of acupuncture to spinal cord injury  anti-scarring, anti-dormant .

Key words:Acupuncture treatment  paraplegia Rabbit  ;Spinal cord neurons cultured in vitro; NGF BFGF MMP ;anti-scarring; anti-dormancy.

[ Last edited by liujie4567 on 2013-3-10 at 12:36 ]

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