| 查看: 144 | 回复: 2 | |||
| 当前主题已经存档。 | |||
| 【悬赏金币】回答本帖问题，作者吴老尚将赠送您 10 个金币 | |||
[求助]
请大家帮忙看看这段英文是否通顺,提出修改意见者金币伺候
|
|||
|
The intrinsic binding constant (K) was obtained by absorption titration . The absorption titration was performed by keeping the concentration of N-(ACR-4-CA)-?-ALA constant while varying the ctDNA concentration. This was done by mixing different volume of ctDNA and the same volume of N-(ACR-4-CA)-?-ALA, and then diluting the mixing solution to 5 ml. This resulted in a series of solutions with varying concentration of ctDNA but with a constant concentration of N-(ACR-4-CA)-?-ALA. K was determined from the plot of D/△?ap vs D. Here D denotes the concentration of ctDNA, △?ap = [?a - ?f] and △??= [?b - ?f]. The apparent extinction coefficient,??a, is obtained by calculating [Aformer - ActDNA - AN-(ACR-4-CA)-?-ALA]/[N-(ACR-4-CA)-?-ALA]. Aforme??r, ActDNA, AN-(ACR-4-CA)-?-ALA denote the absorbance at 261 nm of the former of N-(ACR-4-CA)-?-ALA with ctDNA, the absorbance at 260 nm of ctDNA, and the absorbance at 262 nm of N-(ACR-4-CA)-?-ALA, respectively. ?b, ?f correspond to the extinction coefficient of the bound form of N-(ACR-4-CA)-?-ALA and the extinction coefficient of free N-(ACR-4-CA)-?-ALA, respectively. The data were fitted to equation (2), and K was obtained from the ratio of the slope to the y-intercept. D/△?ap = D/△??+ 1/△?K (2) The intrinsic binding constant of N-(ACR-4-CA)-?-ALA with ctDNA is (3.0±0.5)×105 M-1 in base pairs. That is much larger than N-[2-(dimethylamino)ethyl]acridine-4-carboxamid (7.4×104 M-1) and 9-aminoacridine (7.2×104 M-1) .The result shows that the binding affinity is evaluated as the introduction of ?-alanine on C-9 position of acridine-4-carboxamide. |
回复此楼» 猜你喜欢
求调剂一志愿武汉理工大学材料工程（085601）
已经有7人回复
-
一志愿C9材料与化工专业总分300求调剂
已经有4人回复
-
321求调剂
已经有4人回复
-
307求调剂
已经有4人回复
-
293求调剂
已经有6人回复
-
085602 289分求调剂
已经有3人回复
-
一志愿北京化工大学材料与化工 264分各科过A区国家线
已经有3人回复
-
277分求调剂，跨调材料
已经有3人回复
-
材料292调剂
已经有6人回复
-
材料与化工考研调剂
已经有6人回复
★ ★ ★ ★ ★
吴老尚(金币+5,VIP+0):谢谢!
吴老尚(金币+5,VIP+0):谢谢!
|
我觉得这段文章有以下几个问题（个人意见，水平有限） 1.时态比较乱 2.用词有点重复 3.句子有点短，读起来感觉不连贯。 我稍微修改了一下，因为水平有限，见笑了。 The intrinsic binding constant (K) was obtained by absorption titration [color=]which requires keeping the concentration of N-(ACR-4-CA)-?-ALA constant while varying the ctDNA concentration.This can be done by mixing different volume of ctDNA and the same volume of N-(ACR-4-CA)-?-ALA,then diluting the mixing solution to 5 ml. Thus,a series of solutions with varying concentration of ctDNA but the same concentration of N-(ACR-4-CA)-?-ALA can be got.K is determined from the plot of D/△?ap vs D. Here D is the concentration of ctDNA, △?ap = [?a - ?f] and △??= [?b - ?f]. The apparent extinction coefficient,??a, is obtained through [Aformer - ActDNA - AN-(ACR-4-CA)-?-ALA]/[N-(ACR-4-CA)-?-ALA]. Aforme??r, ActDNA, AN-(ACR-4-CA)-?-ALA denote the absorbance at 261 nm of the former of N-(ACR-4-CA)-?-ALA with ctDNA, the absorbance at 260 nm of ctDNA, and the absorbance at 262 nm of N-(ACR-4-CA)-?-ALA, respectively. ?b, ?f correspond to the extinction coefficient of the bound form of N-(ACR-4-CA)-?-ALA and the extinction coefficient of free N-(ACR-4-CA)-?-ALA, respectively. The data are fitted to equation (2), and K is obtained from the ratio of the slope to the y-intercept. D/△?ap = D/△??+ 1/△?K (2) The intrinsic binding constant of N-(ACR-4-CA)-?-ALA with ctDNA is (3.0±0.5)×105 M-1 in base pairs. That is much larger than that of N-[2-(dimethylamino)ethyl]acridine-4-carboxamid (7.4×104 M-1) and 9-aminoacridine (7.2×104 M-1) .The result shows that the binding affinity is evaluated as the introduction of ?-alanine on C-9 position of acridine-4-carboxamide |
2楼2007-08-25 02:25:45
★ ★ ★ ★ ★
吴老尚(金币+5,VIP+0):谢谢!
吴老尚(金币+5,VIP+0):谢谢!
|
The intrinsic binding constant (K) was determined by absorption titration , which the concentration of N-(ACR-4-CA)-?-ALA was constant while the ctDNA concentration varied. Specifically, A certain volume of N-(ACR-4-CA)-?-ALA was mixed with different volume of ctDNA, then diluted to 5 ml. K was determined from the plot of D/△?ap vs D. Here D denotes the concentration of ctDNA, △?ap = [?a - ?f] and △??= [?b - ?f]. respectively.The apparent extinction coefficient,??a,was calculated by [Aformer - ActDNA - AN-(ACR-4-CA)-?-ALA]/[N-(ACR-4-CA)-?-ALA]. Aforme??r, ActDNA, AN-(ACR-4-CA)-?-ALA are the absorbanceof the complex of N-(ACR-4-CA)-?-ALA with ctDNA at 261 nm , the absorbance of ctDNA at 260 nm , and the absorbance of N-(ACR-4-CA)-?-ALA t 262 nm , respectively. ?b, ?f corresponds to the extinction coefficient of the bound N-(ACR-4-CA)-?-ALA and the extinction coefficient of free N-(ACR-4-CA)-?-ALA, respectively. The data were fitted to equation (2), and K was the ratio of the slope to the y-intercept. D/△?ap = D/△??+ 1/△?K (2) The intrinsic binding constant of N-(ACR-4-CA)-?-ALA with ctDNA is (3.0±0.5)×105 M-1 in base pairs. It is much larger than N-[2-(dimethylamino)ethyl]acridine-4-carboxamid (7.4×104 M-1) and 9-aminoacridine (7.2×104 M-1) .The result shows that the binding affinity is evaluated as the introduction of ?-alanine on C-9 position of acridine-4-carboxamide. 我不知道你想用“evaluated as”说明什么？ 也许能改成increased根据上下文 [ Last edited by langn88 on 2007-8-25 at 06:21 ] |
3楼2007-08-25 06:17:34
