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Neutralizing antibodies against Dll4 and soluble antagonist EphrinB2 were used in vivo to show that VEGF and Dll4 induced EphrinB2, and blockade of Dll4 reduced the induction of EphrinB2 by VEGF. Both anti-Dll4 and soluble EphrinB2 blocked in vivo tumour growth and produced non-productive angiogenesis. These results showed that EphrinB2 played a crucial role in non-productive angiogenesis induced by Dll4 blockade. There are, however, many other angiogenic genes regulated by Dll4. |
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