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Study on the Cellulase Components beta-Endoglucanase Purification by Chromatography ×÷Õß: Nong, X (Nong, Xiang)[ 2 ] ; Wu, H (Wu, Hong)[ 1 ] ; Xu, CH (Xu, Chen-Hao)[ 2 ] ; Fang, CL (Fang, Chun-Lin)[ 3 ] ; Lin, TX (Lin, Tian-Xing)[ 2 ] ; Liang, Z (Liang, Zi)[ 2 ] ; Wang, YY (Wang, Yan-Yun); Qin, TY (Qin, Tian-Ying)[ 1 ] ; Hu, Q (Hu, Qiang)[ 4 ] À´Ô´: ASIAN JOURNAL OF CHEMISTRY ¾í: 25 ÆÚ: 3 Ò³Êý: 1687-1690 ³ö°æÈÕÆÚ: FEB 2013 ±»ÒýÓôÎÊý: 0 (À´×Ô Web of Science) ±»ÒýÓòο¼ÎÄÏ×: 20 [ ¼ìÊÓ Related Records ] ÒýÓÃÎÄÏ×ͼ ÕªÒª: Two active components, EgI and EgII, of beta-endoglucanase were purified by utilizing salting out method, as well as twice Sephadex G-75 and DEAE-Sephadex A-50 chromatography from the fermentation broth of Trichoderma reesei QM9414. The relative molecular weights of EgI and EgII were 65.47 and 57.04 kDa, respectively, as detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The optimal temperatures of EgI and EgII were 50 degrees C and 55 degrees C, respectively and the optimal pH levels were 4.8 and 5.0. The experiment on the effect of different metal ions showed that: K+ could activate EgI and EgII activities and Feb(3+), Cu2+, Zn2+, Ca2+ and Mn2+ were the inhibitors of EgI and EgII. EgI and EgII with good thermal stability, in temperature range of 30-60 degrees C with 0.5 h, EgI and EgII can maintain more than 78 % enzyme activity, EgI and EgII are heat-resistant. The K-m of EgI and EgII was detected using the Lineweaver-Burk plot method, which showed that their Michaelis constants values were 0.238 g/L and 0.266 g/L, yEgI = 0.6078x + 2.5558, yEgII = 0.6665x + 2.5046, respectively. EgI and EgII are new discoveries according to their characterization. Apparently, the present research built a foundation for the further study of beta-endoglucanase in T. reesei QM9414. Èë²ØµÇ¼ºÅ: WOS:000312621400112 ÎļþÀàÐÍ: Article ÓïÑÔ: English ×÷Õ߹ؼü×Ö: Purification; Characterization; Cellulase components; Trichoderma reesei QM9414 KeyWords Plus: ASPERGILLUS-NIGER; GLUCOSIDASE; BINDING; DOMAIN; STRAIN ͨѶ×÷ÕßµØÖ·: Wu, H (ͨѶ×÷Õß)£¬ SW Univ Nationalities, Coll Life Sci, Chengdu 610041, Peoples R China. µØÖ·: [ 1 ] SW Univ Nationalities, Coll Life Sci, Chengdu 610041, Peoples R China [ 2 ] Leshan Normal Univ, Coll Chem & Life Sci, Leshan 614000, Peoples R China [ 3 ] Chengdu Qiankun Vet Pharmaceut Co, Chengdu 611130, Peoples R China [ 4 ] Leshan Bur Qual & Tech Supervis, Leshan, Peoples R China µç×ÓÓʼþµØÖ·: wuhong_0203@126.com ³ö°æÕß: ASIAN JOURNAL OF CHEMISTRY, 11/100 RAJENDRA NAGAR, SECTOR 3,, SAHIBABAD 201 005, GHAZIABAD, INDIA Web of Science Àà±ð: Chemistry, Multidisciplinary Ñо¿·½Ïò: Chemistry IDS ±àºÅ: 058GF ISSN: 0970-7077 ===== µÚ2 ƪ===> ÄúµÄ¼ìË÷ÕÒ²»µ½¼Ç¼¡£ [color]¼ø¶¨Íê±Ï |

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