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seamas_gao2

新虫 (小有名气)

[求助] 请问“Forward primer”和“Upper Oligo”有什么区别?

今天在学习Oligo的用法,发现界面上有两对引物可以选,一对是“Forward Primer和Reverse Primer”,另一对是“Upper Oligo和Lower Oligo”。请问这两对引物有什么区别呢?

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slotsma

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【答案】应助回帖

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seamas_gao2: 金币+5, ★有帮助, 十分感谢! 2013-02-28 16:02:30
"Lower Oligo - a negative strand oligonucleotide that has been selected for
analysis. The Lower Oligo can be manually selected by the user or
automatically selected by OLIGO during TaqMan search."
"Upper Oligo - a positive strand oligonucleotide that has been selected for
analysis. The Upper Oligo can be manually selected by the user or
automatically selected by OLIGO during TaqMan search." 摘自http://www.oligo.net/oligo_7_manual.pdf
意思就是待分析的寡聚核苷酸(候选引物),可以手动选择,也可以由程序搜索得到
3楼2013-01-30 20:47:43
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lidonghong

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【答案】应助回帖

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seamas_gao2: 金币+5, ★有帮助, 十分感谢! 2013-02-28 16:02:16
个人理解,这两对引物应该就是我们通常讲的上下游引物,这个叫法也许是软件自己的讲法。
生物化学与分子生物学
2楼2013-01-30 17:34:12
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seamas_gao2

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引用回帖:
3楼: Originally posted by slotsma at 2013-01-30 20:47:43
"Lower Oligo - a negative strand oligonucleotide that has been selected for
analysis. The Lower Oligo can be manually selected by the user or
automatically selected by OLIGO during TaqMan sear ...

那你的意思是它们是另一组的候选,有第一组了,就不用这一组了是吗
4楼2013-01-31 08:21:34
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seamas_gao2

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有没有人确切知道呢?
5楼2013-01-31 13:29:34
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