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peterretep木虫 (正式写手)
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[求助]
电泳中派若宁Y（Pyronin Y）是什么作用？
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一种荧光染料 Pyronin Y 激发及发射波长555，580 。 哌若宁Y(P·Y)既能与DNA结合也能与RNA结合。 在看文献，做电泳实验，在denaturing sample buffer配方中，用到派若宁Y（Pyronin Y），它是起什么作用的．谢谢。 |
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木虫 (著名写手)
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Pyronin Y solutions are red in transmitted light displaying a yellow fluorescence in reflected light. Pyronin Y is used widely in combination with methyl green to selectively and differentially stain nucleic acids following polyacrylamide gel electrophoresis. In this dichromatic dye solution, the Pyronin Y stains RNA red, while methyl green stains DNA green. Pyronin Y has also be used to quantitate RNA content by flow cytometry, and to stain liver cells fixed in Carnoy's fluid. |
2楼2007-08-06 06:47:55
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木虫 (著名写手)
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3楼2007-08-06 06:49:12
    [ Last edited by wnting on 2007-8-6 at 09:03 ] |
4楼2007-08-06 09:00:51