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blugduaps金虫 (小有名气)
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[交流]
求助:CE-ECL联用
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各位仁兄,大家好! 在下是搞CE-ECL联用的,现有一篇非常重要的文献我们学校的数据库里看不到全文只有摘要,希望哪位好心人帮我找找全文,先谢谢了! S. Douglass Gilman, Charlotte E. Silverman, Andrew G. Ewing * Electrogenerated chemiluminescence detection for capillary electrophoresis Journal of Microcolumn Separations Volume 6, Issue 2 , Pages 97 - 106 Keywords electrogenerated chemiluminescence ?luminol ?capillary electrophoresis ?microelectrodes Abstract Electrogenerated chemiluminescence (ECL) detection based on the reaction of luminol and H2O2 has been developed for capillary electrophoresis. The ECL response at carbon fiber and platinum wire microelectrodes has been investigated. Carbon fiber electrodes are observed to be less sensitive than platinum electrodes but provide a more stable response. The ECL response at a carbon fiber electrode is linear over three orders of magnitude for luminol with a linear correlation coefficient of 0.998. Mass detection limits of 92 amol and 260 amol have been obtained for luminol using platinum wire microelectrodes and carbon fiber microelectrodes, respectively. The influence of hydrogen peroxide concentration (in the detection buffer resrvoir) on the luminescence response has been examined and found to be dependent on electrode type. The dependence of the ECL response on the applied voltage at the microelectrode has been studied for both electrode types. Capillary electrophoresis with ECL detection has been used for analysis of amines derivatized with N-(4-aminobutyl)-N-ethylisoluminol coupled to N, N-disuccinimidylcarbonate (ABEI-DSC). Detection limits of 2.0 fmol and 0.96 fmol are obtained for n-octylamine and n-propylamine, respectively. In addition, ABEI-DSC has been used to successfully label the tripeptide Val-Tyr-Val, and micellar electrokinetic capillary chromatography has been used with ECL detection for the separation of ABEI-DSC labeled amines. [ Last edited by dongfang1629 on 2007-7-26 at 10:23 ] |
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