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求一段英译中，万分感谢
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| Using Surface Plasmon Resonance (SPR) it has been shown that the fine structure of the anionic polysaccharide pectin strongly influences its interfacial interaction with a j-casein layer coated onto a gold surface (via a dextran linker) in the pH range 3.5–6.8, with the highest SPR signal being observed for pectin with the lowest charge density tested (a degree of methylesterification (DM) around 90%).Furthermore, the Brownian motions of j-casein coated polystyrene beads (used to provide calciumfree ‘model casein micelles’) were studied in pectin solutions using Diffusing Wave Spectroscopy (DWS) and microscopy, and were compared with measurements made on naked beads. At every pH value studied (with the exception of 3.5), bridging of the protein-covered probe particles was observed for pectins of both DM 28 and DM 78. However, no aggregated complexes were found in these model casein micelle systems when pectin of an unusually high DM was used (90%). It was hypothesised that having a limited number of binding regions of spatially limited extent maximises the number of chains binding to the protein layer (as found with the SPR measurement), encourages the formation of loops and trains, and additionally limits the potential for destabilisation via bridging. |
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your2007: 金币+30, 翻译EPI+1, ★★★★★最佳答案, 谢谢 2012-12-24 08:48:08
your2007: 金币+30, 翻译EPI+1, ★★★★★最佳答案, 谢谢 2012-12-24 08:48:08
| 使用表面等离子体共振（SPR），已经指出在pH范围3.5-6.8，阴离子多糖果胶的精细结构强烈影响其与覆盖到金表面上的j-酪蛋白层之间的界面相互作用（通过葡聚糖连接器），观察到果胶最高的SPR信号，检测到最低的电荷密度（甲酯化程度（DM）在90％左右）。此外，在果胶溶液中，对被聚苯乙烯珠覆盖的j-酪蛋白的布朗运动（用来提供无钙模型酪蛋白胶束）进行了研究，采用扩散波光谱（DWS）和显微镜，并与裸珠的测量结果进行了对比。在每个pH值（除去3.5），研究果胶DM28和DM78，观察到蛋白质包覆的探针颗粒的桥连。然而，在这些模型酪蛋白体系，当使用一个不寻常的高DM的果胶时（90％），没有发现凝集的配合物。有人假设，空间有限的程度上具有有限数量的桥连区域可以使桥连蛋白质层的链达到最大化（SPR测量发现），鼓励循环和行列的形成，并且还限制了由于桥连不稳定的可能性。 |
2楼2012-12-23 22:04:02
