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关于HPLC分析时色谱条件的选择的一段话 求翻译
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Analytical separation was carried out on a Jasco PV- 1580 HPLC unit using a Supelco 516 C18 column (15 cm × 4.6 mm, 5μm) at room temperature. The detector used was a Jasco UV-1570 UV–vis spectrophotometer set at 254 nm and the volume of sample injected was 20 L. The aqueous phase was prepared by weighing 3.8 g ammonium acetate and dissolving in 1 L water and its pH adjusted to 4.4 using acetic acid. The mobile phase used was methanol–acetate buffer (pH 4.4) (35:65, v/v) for 9 min, after which it was changed to methanol–acetate buffer (pH 4.4) (50:50, v/v). |
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