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yind1014

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[求助] 关于HPLC分析时色谱条件的选择的一段话 求翻译

Analytical separation was carried out on a Jasco PV- 1580 HPLC unit using a Supelco 516  C18 column (15 cm × 4.6 mm, 5μm) at room temperature. The detector used was a Jasco UV-1570 UV–vis spectrophotometer set at 254 nm and the volume of sample injected was 20 L. The aqueous phase was prepared by weighing 3.8 g
ammonium acetate and dissolving in 1 L water and its pH adjusted to 4.4 using acetic acid. The mobile phase used was methanol–acetate buffer (pH 4.4) (35:65, v/v) for 9 min, after which it was changed to methanol–acetate buffer (pH 4.4) (50:50, v/v).
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yind1014: 金币+5, 翻译EPI+1, ★★★★★最佳答案 2012-12-22 15:47:52
分析分离在Jasco PV-1580 液相色谱里进行,室温,Supelco 516 C18 柱子。 探测器是Jasco UV-1570紫外-可见光光谱仪,用254nm,注入样品是20升。水相制备由3.8克醋酸铵溶于一升水,用醋酸调节PH至4.4. 流动相用甲醇-醋酸缓冲液 (pH 4.4) (35:65体积比),之后变为甲醇-醋酸缓冲液(pH 4.4) (50:50体积比).
2楼2012-12-22 15:14:18
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