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| A reversed-phased HPLC method that allows the separation and simultaneous determination of the preservatives benzoic (BA) and sorbic acids (SA), methyl- (MP) and propylparabens (PP) is described. The separations were effected by using an initial mobile phase of methanol–acetate buffer (pH 4.4) (35:65) to elute BA, SA and MP and changing the mobile phase composition to methanol–acetate buffer (pH4.4) (50:50) thereafter. The detector wavelength was set at 254 nm. Under these conditions, separation of the four components was achieved in less than 23 min. Analytical characteristics of the separation such as limit of detection, limit of quantification, linear range and reproducibility were evaluated. The developed method was applied to the determination of 67 foodstuffs (mainly imported), comprising soft drinks, jams, sauces, canned fruits/vegetables, dried vegetables/fruits and others. The range of preservatives found were from not detected (nd)—1260,nd—1390, nd—44.8 and nd—221 mg kg−1for BA, SA, MP and PP, respectively. |
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【答案】应助回帖
★ ★ ★ ★ ★ ★ ★
Carena: 金币+2, 感谢应助 2012-12-21 13:53:45
yind1014: 金币+5, 翻译EPI+1, ★★★★★最佳答案, 非常感谢 跪谢！！！ 2012-12-21 20:37:07
Carena: 金币+2, 感谢应助 2012-12-21 13:53:45
yind1014: 金币+5, 翻译EPI+1, ★★★★★最佳答案, 非常感谢 跪谢！！！ 2012-12-21 20:37:07
| 用于对防腐剂苯甲酸（BA）和山梨酸（SA），尼泊金甲酯（MP），和对羟基苯甲酸丙酯（PP）进行分离和同时测定的反相高效液相色谱的方法被发现。通过用甲醇-乙酸盐缓冲液（pH值4.4）（35:65）作为流动相进行洗脱来实现BA的分离，对于SA和MP的分离则通过将流动相改变为甲醇-醋酸缓冲液（pH4.4）（50： 50）来实现。检测波长设置为254nm。在这些条件下，四种化合物在23min内成功的分离。本文对如检测线、定量限、线性范围和重现性等分析参数做出了评价。所建立的方法适用于测定67种食品（主要是进口的），包括汽水，果酱，调味料，水果/蔬菜罐头，干蔬菜/水果等。对于BA、SA、MP和PP等防腐剂的检测的范围分别是1260ng、1390ng、44.8ng和221mg/kg。 |
2楼2012-12-21 09:10:58
3楼2012-12-21 20:37:33