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[求助]
求4个上市抗癌药物的IC50值
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Currently there are four organic intercalators approved by the FDA for the treatment of human cancers: 1: Daunorubicin (sold under the brand names of Cerubidine® and Daunoxone® and the generic name Daunorubicin hydrochloride) 2: Doxorubicin (sold under the brand name Adriamycin PFS®, Adriamycin RDF®, Rubex® and the generic name Doxorubicin hydrochloride) 3: Mitoxantrone (sold under the brand name Novanthrone ® and its generic name) 4: Amsacrine 求上面4个化合物的对P388和A549两个细胞的IC50值。 请给出具体数值，和文献。万分感谢！ |
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13073456851
金虫 (正式写手)
- 应助: 0 (幼儿园)
- 金币: 1089.8
- 帖子: 472
- 在线: 51.4小时
- 虫号: 252934
- 注册: 2006-05-20
- 性别: GG
- 专业: 药物毒理
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文献摘要一个： Subcellular daunorubicin distribution and its relation to multidrug resistance phenotype in drug—resistant cell line SMMC—7721／R 摘 要:AIM：To investigate the correlation between subcellular daunorubicin distribution and the multidrug resistance phenotype in drug-resistant cell line SMMC-7721/R.METHODS:The multidrug resistant cell line SMMC-7721/R,a human hepatocellular carcinoma cell line,was established.Antisenes oligonucleotides(AS-ODN)were used to obtain different multidrug resistance phenotypes by inhibiting the expression of mdr1 gene and/or multidrug resistance-related protein gene(mrp)using Lipofectamine as delivery agent.Expression of mdr1 and mrp genes was evaluated by RT-PCRand Western blotting.Intracellular daunorubicn(DNR)concentration was measured by flow cytometry.Subcellular DNR distribution was analyzed by confocal laser scanning microscopy.Adriamycin(ADM)and DNR sensitivity was examined by MTTmethod.RESULTS:Low level expression of mdr1 and mrpmRNAs and no expression of P-Glycoprotein(P-gp)and multidrug resistance-related protein(P190)were detected in parental sensitive cells SMMC-7721/S,but over-expression of these two genes was observed in drug-resistant cell SMMC-7721/R,The expression of mdr1 and mrp genes in SMMC-7721/Rcells was down-regulated to the level in the SMMC-7721/Scells by AS-ODN.Intracellular DNAconcentration in SMMC-7721/Scells was 10times higher than that in SMMC-7721/Rcells.In SMMC7721/Scells intracellular DNA distributed evenly in the nucleus and cytoplasm.while in SMMC-7721/Rcells DNR distributed in a punctate pattern in the cytoplasm and was reduced in the nucleus.DNR concentration in SMMC-7721/Rcells co-transfected with AS-ODNs targeting to mdr1and rpmRNAs recovered to 25percent of that in SMMC7721/Scells.Intracellular DNA distribution pattern in drug-resistant cells treated by AS-ODN was similar to drug-sensitive cell.and the cells resistance index(RI)to DNA and AMD decreased at most from 88.0and 116.0to4.0and 2.3,repectively.Co-Transfection of two AS-ODNs showed a stronger synergistic effect than separate transfection.CONCLUSIONS  -gp and P190are two members mediatingMDR in cellline SMMC7721/R,Intracellular drug concentration increase and subcellular distribution change are two important factos in multidrug resistance(MDR)formation.The second facto,drugs transport by P-gp andP190from cell nucleus to organell in cytoplasm,may play a more important role. |
2楼2007-07-18 17:03:13
13073456851
金虫 (正式写手)
- 应助: 0 (幼儿园)
- 金币: 1089.8
- 帖子: 472
- 在线: 51.4小时
- 虫号: 252934
- 注册: 2006-05-20
- 性别: GG
- 专业: 药物毒理
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SOMATOSTATIN MAY ENHANCE CYTOTOXIC EFFECT OF DOXORUBICIN ON GALLBLADDER CANCER CELLS李济宇[1] 全志伟[1] 张强[2] 刘建文[2][1]DepartmentofGeneralSurgery,XinhuaHospital,ShanghaiSecondMedicalUniversity,Shanghai200092 [2]StateKeyLaboratoryofBioreactorEngineering,NewWorldInstituteofBiotechnology,EastChinaUniversityofScienceandTechnology,Shanghai200237摘 要:Objective: To explore the change of chemosensitivity of gallbladder cancer cells pre-treated with somatostatin. Methods: Twenty-four hours after somatostatin treatment, gradient concentrated Doxorubicin was added and growth curve of gallbladder cancer cells was investigated to measure IC50, i.e.,concentration of l)oxorubicin at 50% cell viability.Results: Somatostatin ccould induce gallbladder cancer cell growth arrest in S phase. Inhibition of growth of cancer cell line was detected by Doxorubicin concentration- dependently (P<0.05). IC50 value was significantly lower by combined-treating with somatostatin and Doxorubicin compared with by Doxorubicin alone (P<0.05). Conclusion: Somatostatin could increase the cytotoxic effect of Doxorubicin on gallbladder cancer cell by modulating its cell cycle. 关键词:生长激素抑制素 增强作用 细胞毒素 阿霉素 胆囊癌 癌细胞 消化系统 分类号: R735.8文献标识码：文章编号：栏目信息：Articles 相关文献:主题相关 |
3楼2007-07-18 17:04:59