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沈阳药科大学天然药化实验讲义
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| 介绍天然药物化学本科实验的操作内容及注意事项 |
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Sephadex™ LH-20 Technical InformationTECHNICAL SPECIFICATIONS Matrix Hydroxypropylated, cross-linked dextran Bead form Spherical, porous Particle size range (diameter) dry 18-111 µm in methanol 27-163 µm pH stability 2-13 (working and cleaning) Chemical stability Stable in most aqueous and organic eluent systems. Not stable below pH 2 nor to strong oxidising agents. Autoclavable 20 min at 121°C TECHNICAL SPECIFICATIONS Maximum linear flow rate ~12 cm/min Recommended linear flow rate ~1 cm/min but depends on resolution required Operating temperature 4°C to 40°C Sample loading volumes adsorption mode determined by desired degree of resolution Molecular sizing ~2% total bed volume Partition mode ~1% total bed volume Exclution limit* peptides ~4 kDa, small organics ~5 kDa Shelf-life 5 years Recommended storage Maintain dry at 4°C to 25°C, wet 4°C to +8°C, pH 6-8, with bacteriostat * Depends on solvent in which the medium has been equilibrated. 以上是Sephadex™ LH-20的技术参数， 其中的Maximum linear flow rate ~12 cm/min Recommended linear flow rate ~1 cm/min but depends on resolution required cm/min 按照GE公司说的是每分钟流过的柱高是否正确？ |
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