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芒辰

金虫 (初入文坛)

[求助] 基因敲除的细胞系如何获得

我有一株正常细胞 想获得其中某一种基因敲除的细胞系 要能稳定传代的  想求问具体过程
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刘玉佩

金虫 (小有名气)

【答案】应助回帖

你是做什么细胞?你要的是稳转的基因敲除细胞系,只能用基因敲除了,具体原理是同源重组,但是方法有很多,Cre-Loxp/Talen/IOS,你可以了解一下
3楼2012-12-31 15:02:45
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zizhuxuan

金虫 (正式写手)

【答案】应助回帖

感谢参与,应助指数 +1
可用限制性核内切酶酶切
2楼2012-11-02 21:46:55
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