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The impact of LCFA adsorptio n on the metha nogenic activity was evaluated in batch assays for two anaerobic granular sludges in the presence and absence of bentonite as synthetic adsorbent. A clear inhibitor y effect at an oleate (C18:1) concentration of 0.5g C18:1L/1 was observed for both sludges. Palmitate (C16:0) was confirme d to be the main intermediate of C18:1 deg radation in not adapted sludge and its accumulatio n was further evidenced by fluorescence staining and micr oscopy techniques. LCFA inhibition could be decreased by the additio n of ben tonite, reducing the lag-phase and accelerating the kinetics of LCFA degradation, concluding in the importance of the adsorptive nature of the LCFA inhibitory process. Granule morphology and molecular profiling of predominant microorganisms revealed that biomass adaptat ion to LCFA could modify the intermediates accumulation profiles and process rates. |
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