| 查看: 1303 | 回复: 3 | ||
[求助]
请问大家烷基磷酸酯跑TLC板时用什么显色剂合适
|
如题，化合物如图 ]RSKHX3VE0}[[$UV(4WQ_{Y.jpg |
回复此楼» 猜你喜欢
» 本主题相关价值贴推荐，对您同样有帮助:
TLC点板时，为什么点浓度大些显色时原点也有颜色呢？
已经有15人回复
- 请各位大侠帮忙……TLC显色剂问题！！！！
已经有11人回复
- TLC跑板显色问题
已经有19人回复
- TLC检测时，羧酸用什么显色剂？
已经有5人回复
- TLC展开剂显色剂
已经有9人回复
- TLC 显色剂求助
已经有6人回复
- 这个物质点板子用什么显色剂？
已经有11人回复
- 【求助】TLC显色剂的选择
已经有9人回复
- 【求助】正辛基丙二酸二乙酯和正十二烷基丙二酸二乙酯的TLC显色剂用什么好？
已经有20人回复
- 【求助】脂肪按类TLC显色剂问题
已经有5人回复
- 【求助】多元醇跑板用做什么显色剂
已经有9人回复
2楼2012-10-08 15:03:04
3楼2012-11-05 10:02:32
netscaner
木虫 (正式写手)
- 应助: 237 (大学生)
- 金币: 8270.5
- 散金: 20
- 红花: 16
- 帖子: 631
- 在线: 87.8小时
- 虫号: 242727
- 注册: 2006-04-11
- 专业: 植物资源学
【答案】应助回帖
|
这个也许可以在荧光下看暗斑点。 以下虽说可以显磷酸酯，但是磷钼酸是一个通用显色剂，不算专属。 Ammonium molydbate For detection of phosphoric acid derivatives Solution I: 1M perchloric acid in water/acetone (1:1) Solution II: ammonium molybdate soln: 5g (NH4)6Mo7O24.4 H2O in 35ml semi-conc. Nitric acid and 65ml water. Solution III: Tin(II) chloride soln: 0.5g SnCl2.2 H2O in 100ml 0.5M hydrochloric acid: Dry developed chromatogram and heat to 60 C Hydrolyse di- and triphosphates by spraying perchloric acid (solution I) onto the warm plate. After spraying 2 times, dry plate slowly at 50 C. Amidophosphates might not be decomposed. In any case, spray the still warm plate with ammonium molybdate solution (solution II) Then spray the still wet plate with tin (II) chloride solution (solution III) Results: Phosphates appear as blue to blue-green spots. Polyphosphates can also be detected by dipping the plates in a solution of ammonium molybdate (1g) dissolved in water (8ml) and perchloric acid (3ml, ca. 70%),filled up to 100ml with acetone. Then phosphates appear as yellow-green spots on a blue background. Also see Molybdenum blue reaction according to Dittmer and Lester. |
4楼2012-11-05 15:47:41
