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LC_S.pdf: http://www.divshare.com/download/929457-736 BOOK 3 Chrom-Ed Book Series Raymond P. W. Scott LIQUID CHROMATOGRAPHY CONTENTS Introduction....................................................................................... 1 The Basic Liquid Chromatograph..................................................... 2 The Gradient Programmer and the LC Pump ............................... 4 The Gradient Programmer ........................................................ 4 The LC Pump................................................................................ 6 The Pneumatic Pump ................................................................ 7 Non-Return Valves ................................................................... 8 The Syringe Pump .................................................................... 9 The Single Piston Reciprocating Pump ...................................... 11 The Rapid Refill Pump ........................................................... 12 The Twin-Headed Pump. ........................................................ 13 The Diaphragm Pump............................................................. 15 The Sample Valve....................................................................... 16 Column Switching .................................................................. 19 Column Ovens ................................................................................ 20 Detectors ......................................................................................... 21 The UV Detector......................................................................... 21 The Fixed Wavelength Detector ................................................. 23 The Multi-Wavelength Detectors ............................................... 25 The Multi-Wavelength Dispersive Detector........................... 26 The Diode Array Detector .......................................................... 27 The Electrical Conductivity Detector ............................................. 29 The Fluorescence Detector ............................................................. 32 The Refractive Index Detector........................................................ 34 The Tridet Multi Functional Detector............................................. 36 Liquid Chromatography Stationary Phases .................................... 41 Silica Gel..................................................................................... 41 The Preparation of Spherical Silica Gel ................................. 43 The Structure of Silica Gel ..................................................... 44 The Thermogravimetric Analysis of Silica Gel...................... 47 Bonded Phases ............................................................................ 49 The Synthesis of Bonded Phases ............................................ 49 Bonded Phase Synthesis by Reaction in a Solvent................. 50 The Fluidized Bed Method for Bonded Phase Synthesis ....... 53 Choosing a Bonded Phase........................................................... 56 Types of Bonded Phase............................................................... 57 Oligomeric Phases .................................................................. 58 Bulk Phases............................................................................. 59 Interactions Between 'Brush' and 'Bulk' Reverse Phases and Aqueous Solvents ................................................................... 60 The Retention Properties of Bulk and Brush Phases...................... 63 Macroporous Polymers ................................................................... 66 LC Mobile Phases ........................................................................... 68 Solvent/Solute Interactions with the Silica Gel Surface............. 69 Solute Stationary Phase Interactions ...................................... 71 Solvent/Solute Interactions with the Reversed Phase Surface ... 74 Molecular Interactions in the Mobile Phase ............................... 76 Aqueous Solvent Mixtures.......................................................... 78 Macrocyclic Glycopeptide Phases .............................................. 82 Cyclodextrin................................................................................ 89 Liquid Chromatography Applications ............................................ 93 References..................................................................................... 101 [ Last edited by 佳怡 on 2009-4-22 at 14:28 ] |
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