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【答案】应助回帖
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prospect087508: 金币+30, 翻译EPI+1, 26 2012-09-19 08:49:27
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Preparation of 2.5% sodium alginate solution, 121 ℃ sterilization 15 min, cooling to 20 ℃, and the activation of active dry yeast after mixed, with syringe drop added to 2.0% CaCl2 solution granulation 30 minutes, with sterile water flushing immobilized yeast, back to 2.0% CaCl2 solution fixed 24 h. Say take after pretreatment of wheat straw, initial substrate concentration for 80 g/L, add a certain amount of medium solution, cellulose enzyme fluid and surface active agent, in a certain initial pH, temperature, stirring speed conditions preliminary enzyme solution. 12 h trailing material, make the substrate final concentration to 120 g/L, and add a certain amount of immobilized yeast cells, adjust pH 5.0, mix well with plastic wrap and let after sealing, put table in shock, 84 h after fermentation end, sampling measurement ethanol production. All test results in 250 ml bottle fermentation, every processing repeat 3 times. |
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看着都好难，顶一下吧