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ÓÐÁË£¡ Comparison of Lipogenesis Capacity of Grass Carp (Ctenopharyngodon idella) Preadipocyte from Different Anatomical Sites and Body Weight Author(s): Li, Y (Li, Ying)1; Gao, Y (Gao, Yang)1; Zhang, YJ (Zhang, Yujuan)1; Hao, YJ (Hao, Youjin)1 Source: JOURNAL OF ANIMAL AND VETERINARY ADVANCES Volume: 11 Issue: 3 Pages: 375-380 Published: 2012 Times Cited: 0 (from Web of Science) Cited References: 18 [ view related records ] Citation Map Abstract: Fat cells in adipose tissue turn over throughout the life span. The objective of this study was to explore the cellular and molecular basis for regional and body weight differences in grass carp (Ctenopharyngodon idella) preadipocyte. The number of fresh isolated preadipocytes were counted from different body weight and anatomical sites using the hemocytometer and the proliferation capacity of the cells was tested by MTT assay. In addition, the differentiation capacity of cells was assessed through mRNA expression of Peroxisome Proliferator-Activated Receptor-gamma ((PPAR-gamma) and cellular Triglycerides (TG) content. The total number and proliferative capacity of visceral cells was significantly (p<0.001) higher compared with cells in the back subcutaneous and there was a very significant (p<0.001) decline in the proliferative capacity of cells with the increase of body weight. The highest TG value and mRNA level of PPAR gamma were obtained in the visceral cells from 5000 g fish and the TG value, mRNA level of PPAR? in cells increased to a greater extent with the increase of fish body weight, irrespective of the depot origin. Moreover, in the case of the same weight of fish, there was a greater differentiation degree in visceral cells than in back subcutaneous cells. In summary, preadipocyte lipogenic capacity of growing fish depends mainly on fat depot origin and significantly greater lipogenic capacity of the fish's adipose tissue is localized visceral. Furthermore, the proliferation capacity of fat cell declines with the increase of body weight and the increasing weight during the growth of healthy fish enhances the lipogenic capacity. Researchers conclude that fat cells from different regions and body weight are inherently distinct. Accession Number: WOS:000298100200016 |
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2Â¥2012-09-16 19:44:53
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Document Type: Article Language: English Author Keywords: Anatomical sites; body weight; preadipocyte; lipogenesis capacity; grass carp; China KeyWords Plus: ENERGY-METABOLISM; GENE-EXPRESSION; DEPOT ORIGIN; FAT; ADIPOGENESIS; ADIPOCYTE; ASSAY; AGE Reprint Address: Li, Y (reprint author), Chongqing Normal Univ, Coll Life Sci, Huxi Campus, Chongqing 401331, Peoples R China. Addresses: 1. Chongqing Normal Univ, Coll Life Sci, Chongqing 401331, Peoples R China Funding: Funding Agency Grant Number National Natural Science Foundation of China 30800843 [Show funding text] Publisher: MEDWELL ONLINE, ANSINET BUILDING, 308-LASANI TOWN, SARGODHA RD, FAISALABAD, 38090, PAKISTAN Web of Science Categories: Veterinary Sciences Research Areas: Veterinary Sciences IDS Number: 862NS ISSN: 1680-5593 |
3Â¥2012-09-16 19:45:50
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4Â¥2012-09-16 20:01:46
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5Â¥2012-09-16 22:29:21













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